The Needs and Applications of Delivery Systems to Fortify Food with Active Ingredients
Chunqing Bai, Yangkai Guo, Lili Chen and Li Zhao*
National R&D Branch Center for Freshwater Fish Processing, Jiangxi Science and Technology Normal University, China
Submission: January 13, 2021;Published: January 28, 2021
*Corresponding author: Li Zhao, National R&D Branch Center for Freshwater Fish Processing, College of Life Science, Jiangxi Science and Technology Normal University, Nanchang 330013, China
How to cite this article: Chunqing Bai, Yangkai Guo, Lili Chen, Li Zhao. The Needs and Applications of Delivery Systems to Fortify Food with Active Ingredients. Nutri Food Sci Int J. 2021. 10(4): 555792. DOI:10.19080/NFSIJ.2021.10.555792.
Abstract
Fortifying food products with bioactive agents is a major initiative within the food industry for human health. However, many agents are water insoluble with low bioavailability; some are unstable and easily degrade during storage. Thus, it is necessary to overcome these obstacles before adding them to the final food products. Entrapping bioactive agents into suitable delivery systems such as liposomes, emulsions, etc., has been proven to be a useful method. This review simply describes the reasons for bioactive agents needed to be entrapped in delivery systems, the common formulations used, and their applications. This would provide a general understanding of how active ingredients generally enrich food.
Keywords: Active ingredients; Delivery systems; Emulsion; Liposome
Introduction
Consumers are increasingly demanding a healthy diet rich in active ingredients such as flavors, vitamins, minerals, antioxidants, lipids and so on [1-3]. However, on the one hand, the compositions of active ingredients in natural food are usually limited; on the other hand, some agents may be high in raw food, but their bioavailability is very low [4-6]. For example, β-carotene has received increasing attention during the past 30 years due to its beneficial health effects such as antioxidant and anticancer activities as well as its ability to reduce the risk of heart disease and certain chronic disease [7]. However, humans cannot synthesize β-carotene in their bodies, and they must obtain it from the foods they consume. Many vegetables and fruits (peppers, tomatoes, carrots, mangoes, and kale) are rich in β-carotene, but literatures show that only a minor part (<10%) of the carotenoids in these raw foods is absorbed [8,9]. In addition, many active ingredients including β-carotene also have many other disadvantages such as low water-solubility, physical or chemical instability, undesirable flavor, and so on [10-12]. All of them make active ingredients difficult to directly incorporate into final food products. Therefore, development of strategies to incorporate active ingredients is necessary if such foods are to keep successful and initiative in the marketplace.
Luckily, these challenges can be overcome by entrapping active ingredients into suitable delivery systems. During the past decades, delivery systems (such as liposomes, emulsions, lipid particles, microcapsules, beads, etc.) have been effectively designed and utilized in food industry to encapsulate, protect, and deliver functional components before introduced into final food products [13-16].
Among of them, emulsions especially for oil-in-water emulsion have attracted the interest of many research groups in food and pharmaceutical fields due to their favorable properties such as good biocompatibility, easy design and preparation [3,17]. To obtain uniform emulsion, the oil phase dissolved with bioactive agents is mixed with emulsifier water solution, blended and then passed through a homogenizer. In this system, emulsifiers play an important role in the formation of emulsion and its stability thereafter. To be an effective emulsifier, ingredients should exhibit perfect emulsifying activity [18]. That means it should quickly adsorb to the surfaces of small oil droplet form an interfacial coating and appreciably decrease the interfacial tension during homogenization [3]. Synthetic emulsifiers such as Tween 20, tween 80 are the traditional emulsifiers for many products. With the increasing demand for food and beverage with natural ingredients in recent years, many food manufacturers are trying to replace synthetic emulsifiers with natural and sustainable alternatives. To date, researchers have found a lot of natural emulsifiers (such as protein, polysaccharides, phospholipids, and bio surfactants), which are able to form and stabilize oil droplets [18-21]. Chung et al., [18] found that emulsion prepared by using quillaja saponin as a natural emulsifier could produce whitish milk similar to a commercial liquid creamer. Lin et al., [22] have prepared β-carotene loaded emulsion by using modified starch. He et al., [4] have fabricated curcumin emulsions containing Konjac glucomannan stabilized with whey protein isolate and achieved a controlled and sustainable release of bioactive compounds from emulsions. Lv et al., [20] have compared the effectiveness of a number of natural emulsifiers (whey protein, gum arabic and quillaja saponin) on the production and stability of vitamin E fortified emulsions and found that whey protein isolate and quillaja saponin were more effective at forming emulsions containing fine droplets than gum arabic, while quillaja saponin based and gum arabic based emulsions exhibited better resistance to pH change. Due to the above-mentioned advantages, emulsions are already widely utilized in the food industry, e.g., in dressings, sauces, soups, beverages, dips, creams, and desserts [6,19,22].
Liposome, as one of the most common used formulations, also has great potential to embed bioactive agents. A liposome is a self-assembling and cell-resembling delivery system with concentric bilayer structure. The size of liposome is typically about 10 to 1000nm with the structure varying from a balloonlike unilamellar to an onion-like multiple structures [3,12]. There are different hydrophilic and hydrophobic regions in liposomes separated by surfactant substances (such as phospholipids) [3]. Therefore, both hydrophilic and lipophilic molecules and even amphiphilic molecules can all be entrapped in the special bilayer. Since liposomes were first described in the 1960s by Alec Bangham, they have been beneficial in medical, cosmetic, and agricultural fields, and have become integral to food research. More recently, liposomes have been widely used to encapsulate proteins/peptides/enzymes, polyphenols/flavones, essential oils/fatty acids, vitamins, energetic substrates, and minerals [3,23-25]. Active agents in the form of liposomes could then be effectively added into different food products: dairy products (milk, cheese, and yogurt), drinks (juices and milk drinks), meat (pork, beef, and rabbit), and other products (chocolate, tofu, etc.) [12,27-32].
Delivery systems containing bioactive agents could be directly added into food material with simple blending or homogenization and bring a number of benefits to food industry: supplying good protection for bioactive agents against environment stress, masking off-flavor (bitterness, astringency), improving storage and handling characteristics, extending shelf life, besides increasing bioavailability. However, the food system microenvironment (pH, intrinsic component, and ionic strength etc.) and emulsifier properties used are critical factors needed to take into account if appropriate final food is wanted [3,6]. For example, whey proteinbased emulsion could effectively inhibit oxidation of fish oil in milk, but is less stable in yoghurt and dressing [18].
Conclusion
A number of challenges associated with active ingredient need to be addressed when incorporated into food. Suitable delivery systems such as emulsions, liposomes, etc. could be designed to encapsulate active agents followed by simple blending or homogenization. However, whether appropriate final food could be obtained is dependent on food and emulsifier properties.
References
- Araiza-Calahorra A, Akhtar M, Sarkar A (2018) Recent advances in emulsion-based delivery approaches for curcumin: From encapsulation to bio accessibility. Trends in Food Science and Technology 71:155-169.
- Fathi M, Donsi F, McClements DJ (2018) Protein-Based Delivery Systems for the Nanoencapsulation of Food Ingredients. Comprehensive Reviews in Food Science and Food Safety 17(4): 920-936.
- McClements DJ (2014) Nanoparticle- and microparticle-based delivery systems: encapsulation, protection and release of active compounds. CRC Press, Boca Raton, FL, USA, pp. 7-22.
- He S, Gu CH, Wang D, Xu W, Wang R, et al. (2020) The stability and in vitro digestion of curcumin emulsions containing Konjac glucomannan. LWT-Food Science and Technology 117: 108672.
- Huang Q, Yu H, Ru Q (2010) Bioavailability and delivery of nutraceuticals using nanotechnology. Journal of Food Science 75(1): 50-57.
- McClements DJ, Decker EA, Park Y, Weiss J (2009) Structural design principles for delivery of bioactive components in nutraceuticals and functional foods. Critical Reviews in Food Science and Nutrition 49(6): 577-606.
- Ha DO, Chan UP, Kim MJ, Lee JH (2012) Antioxidant and prooxidant activities of β-carotene in accelerated autoxidation and photosensitized model systems. Food Science and Biotechnology 21(2):607-611.
- Kaulmann A, Bohn T (2014) Carotenoids, inflammation, and oxidative stress-implications of cellular signaling pathways and relation to chronic disease prevention. Nutr Res 34(11): 907-929.
- Bai CQ, Zhen JX, Zhao L, Chen LL, Xiong H, et.al. (2019) Development of oral delivery systems with enhanced antioxidant and anticancer activity: Coix seed oil and β Carotene co-loaded liposomes. Journal of agricultural and food chemistry 67(1): 406-414.
- Liu W, Wang J, McClements DJ, Zou L (2018) Encapsulation of β-carotene-loaded oil droplets in caseinate/alginate microparticles: Enhancement of carotenoid stability and bioaccessibility. Journal of Functional Foods 40: 527-535.
- Wang P, Liu HJ, Mei XY, Nakajima M, Yin LJ (2012) Preliminary study into the factors modulating β-carotene micelle formation in dispersions using an in vitro digestion model. Food Hydrocolloids26(2): 427-433.
- Liu W, Ye A, Han F, Han J (2019) Advances and challenges in liposome digestion: Surface interaction, biological fate, and GIT modeling. Advances in Colloid and Interface Science 263: 52-67.
- Zhang R, McClements DJ (2016) Enhancing nutraceutical bioavailability by controlling the composition and structure of gastrointestinal contents: Emulsion-based delivery and excipient systems. Food Structure 10: 21-36.
- McClements DJ (2017) Recent progress in hydrogel delivery systems for improving nutraceutical bioavailability. Food Hydrocolloids 68: 238-245.
- Wu W, Lu Y, Qi J (2015) Oral delivery of liposomes. Therapeutic Delivery 6(11): 1239-1241.
- Zhang Z, Zhang R, McClements DJ (2017) Control of protein digestion under simulated gastrointestinal conditions using biopolymer microgels. Food Research International 100: 86-94.
- Ji N, Hong Y, Gu Z, Cheng L, Li Z, et.al. (2018) Preparation and characterization of insulin-loaded zein-carboxymethylated short-Chain amylose complex nanoparticles. Journal of Agricultural and Food Chemistry 66(35: 9335-9343.
- Chung C, Sher A, Rousset P, Decker EA, McClements DJ (2017) Formulation of food emulsions using natural emulsifiers: Utilization of quillaja saponin and soy lecithin to fabricate liquid coffee whiteners. Journal of Food Engineering 209: 1-11.
- Let MB, Jacobsen C, Meyer AS (2007) Lipid oxidation in milk, yoghurt, and salad dressing enriched with neat fish oil or pre-emulsified fish oil. Journal of Agriculture and Food Chemistry 55(19): 7802-7809.
- Lv S, Zhang Y, Tan H, Zhang R, McClements DJ(2019) Vitamin E encapsulation within oil-in-water emulsions: Impact of emulsifier type on physicochemical stability and bioaccessibility.J Agric Food Chem 67(5):1521-1529.
- Amagliani L, O'Regan J, Kelly AL, O'Mahony JA (2017) The composition, extraction, functionality and applications of rice proteins: A review. Trends in Food Science & Technology 64:1-12.
- Lin Q, Liang R, Ye A, Singh H, Zhong F (2017) Effects of calcium on lipid digestion in nanoemulsions stabilized by modified starch: Implications for bioaccessibility of b-carotene. Food Hydrocolloids 73: 184-193.
- Liu WL, Liu W, Liu CM, Yang SB, Liu JH, et.al. (2011) Medium-chain fatty acid nanoliposomes suppress body fat accumulation in mice. The British Journal of Nutrition 106(9): 1330-1336.
- Tai K, Rappolt M, He X, Wei Y, Zhu S, et.al. (2019) Effect of β-sitosterol on the curcumin-loaded liposomes: Vesicle characteristics, physicochemical stability, in vitro release and bioavailability293: 92-102.
- Marín D, Alemán A, Sánchez-Faure A, Montero P, Gómez-Guillén MC (2018) Freeze-dried phosphatidylcholine liposomes encapsulating various antioxidant extracts from natural waste as functional ingredients in surimi gels. Food Chemistry 245: 525-535.
- Malheiros PS, Cuccovia IM, Franco BDGM (2016) Inhibition of Listeria monocytogenes in vitro and in goat milk by liposomal nanovesicles containing bacteriocins produced by Lactobacillus sakei subsp. sakei 2a. Food Control 63:158-164.
- Toniazzo T, Berbel IF, Cho S, Favaro-Trindade CS, Moraes ICF (2014) β -carotene-loaded liposome dispersions stabilized with xanthan and guar gums: Physico-chemical stability and feasibility of application in yogurt. LWT-Food Science and Technology 59: 1265-1273.
- Cui HY, Wu J, Lin L (2016) Inhibitory effect of liposome-entrapped lemongrass oil on the growth of Listeria monocytogenes in cheese. Journal of Dairy Science 99(8): 6097-104.
- Liu W, Tian M, Kong Y, Lu J, Li N, et.al. (2017) Multilayered vitamin C nanoliposomes by self-assembly of alginate and chitosan: long-term stability and feasibility application in mandarin juice. LWT- Food Science and Technology 75: 608-15.
- Marsanasco M, Calabró V, Piotrkowski B, Chiaramoni NS, del VAlonso S (2016) Fortification of chocolate milk with omega-3, omega-6, and vitamins E and C by using liposomes. European Journal of Lipid Science and Technology 118(9): 1271-1281.
- Kim JM, Kim YJ, Jeong J, Kim CJ (2006) Meat tenderizing effect of injecting encapsulated Ca2+ in liposome into rabbit before slaughter. Bioscience Biotechnology and Biochemistry 70:2381-2386.
- Kong B, Zhang H, Xiong YL (2010) Antioxidant activity of spice extracts in a liposome system and in cooked pork patties and the possible mode of action. Meat Science 85(4): 772-778.