Radiotracer Assay of Microbial Processes in Petroleum Reservoirs
Tamara N Nazina*, Natalya M Shestakova, Valeriy S Ivoilov, Nadezda K Kostrukova, Sergey S Belyaev and Mikhail V Ivanov
Department of Microbial Biogeochemistry and Biogeotechnology, Winogradsky Institute of Microbiology, Russia
Submission: February 17, 2017; Published: March 14, 2017
*Corresponding author: Tamara N Nazina, Winogradsky Institute of Microbiology, Research Center of Biotechnology, Russian Academy of Sciences, prospect 60-letiya Oktyabrya, 7/2 Moscow, 117312, Russia, Tel: +7-499-135-03-41; Email: nazina@inmi.ru
How to cite this article: Nazina T N,Shestakova N M, Ivoilov V S, Kostrukova N K, BelyaevS S, et al. Radiotracer Assay of Microbial Processes in Petroleum Reservoirs. Adv Biotech & Micro. 2017; 2(4): 555591. DOI: 10.19080/AIBM.2017.02.555591
Abstract
The paper provides quantitative characterization of the rates of aerobic (methane oxidation) and anaerobic (sulfate reduction, methanogenesis, acetogenesis) biogeochemical processes in oilfields with various physicochemical and hydrodynamic conditions and type of oil-bearing rocks determined using radioactive labeled substrates. Importantly, detection of a specific physiological group of microorganisms by cultural or molecular techniques does not automatically imply bacterial activity in environmental samples. For reliable detection of contemporary biogeochemical processes, a combination of radioisotope methods with physicochemical, biogeochemical, microbiological, and molecular biological techniques is required.
Keywords:Petroleum reservoirs; Methanogenesis; Acetogenesis ; Sulfate reduction; Radioisotope tracing methods
Introduction
Microbial communities of petroleum reservoirs are among the most ancient biocenoses on Earth. The study of these communities is necessary to gain knowledge on the evolution of the biosphere and on the genesis and transformation of petroleum hydrocarbons. Little is known concerning deep subterranean flows of matter.
Oilfield microbial communities exist under conditions of hampered exchange of water and mass and at a constant temperature; they are independent of the modern atmosphere and sunlight and may be considered closed or semi-closed systems. Crude oil is the main source of organic matter in this ecosystem. Microbial growth in reservoirs can be limited by the low content of phosphate and ammonium nutrients [1,2]. Formation waters usually contain no dissolved oxygen and nitrate, while sulfate and other oxidized sulfur compounds are present, as well as carbonate. Sulfate reduction, methanogenesis, and acetogenesis are therefore probably the major metabolic processes occurring in such ecosystems. Since microbial processes are very slow due to low water exchange and scarcity of biogenic elements, oil in the reservoirs is preserved for geological periods. Activation of water exchange resulting from geological processes or anthropogenic activity returns the buried organic matter of petroleum into the biogeochemical cycles. Present-time biogenic processes of petroleum transformation and the relevant microorganisms are relatively poorly studied.
Radiotracer techniques may be used for integral assessment ofthe activity ofa microbial community as a whole. The techniques for determination of the rates of terminal stages of petroleum biodegradation (sulfate reduction and methanogenesis) using radioactively labeled Na235SO4, NaH14CO3, and 14CH3COONa have been developed by Russian researchers - Ivanov MV (1956) [3], Belyaev SS [4,5] and co-workers. Introduction of radiolabeled substrates (35SO42-, 14CO2, 14CH3COOH) into formation water results in synthesis of labeled radioactive products and makes it possible to assess the rates of the relevant processes:
The aim of this work was to summarize the results of quantitative assessment of microbial geochemical activity in petroleum reservoirs with different physicochemical conditions and collector properties. Rozanova et al. [6] were the first who used radioisotope technique to measure the rate of sulfate reduction in petroleum reservoirs. Quantitative characteristics of anaerobic biogenic processes in petroleum reservoirs are presented in Table 1. Sulfate reduction and methanogenesis were registered by radioisotope methods in the layers with temperatures from 17 to 80 °C and salinity of up to 287g L-1. In petroleum reservoirs exploited with water-flooding, the rates of both processes were highest in the water from the near-bottom zone of injection wells; they were therefore concentrated in the zone of petroleum oxidation (Table 1). Our results demonstrate that in water-flooded petroleum reservoirs, both aerobic- anaerobic and anaerobic processes are important in petroleum biodegradation [7-11].
*Rate in water from the near-bottom zone of injection well; other data apply to formation water from production wells. ** Rate at 80 °C. Nd -no data
Rates of microbial processes in petroleum reservoirs with carbonate oil-bearing rocks
The major role of bacterial sulfate reduction in sulfide formation in carbonate petroleum collectors containing sulfate is presently established [12-15]. For example, sulfate reduction was the dominant terminal process in the Bavlinskoe oilfield and in beds 302 and 303 of the Romashkinskoe oilfield, where the levels of sulfate and sulfide in the water are high; its rate reached 171-237μg S2-L-1 day'1, while the rate of methanogenesis was 10100 times lower [13-15]. Sulfate reduction was limited by the absence of available organic substrates and biogenic elements (nitrogen, phosphorus, and iron). Amendment of isolated formation water samples with hydrogen, acetate, benzoate, hexadecane, or especially methane resulted in stimulation of sulfate reduction (Figure 1) [9]. Thus, apart from H2/CO2, lower alcohols, and volatile fatty acids, hexadecane and methane can also act as carbon and energy sources for sulfidogenic communities of the oil reservoirs with carbonate collectors. This was confirmed by obtaining sulfidogenic enrichments on these substrates and on molasses [9,16].
Sulfate-reducing prokaryotes identified in the water samples from carbonate and terrigenous oilfields are listed by Gieg et al. [17]. The range of substrates utilized by sulfidogenic microbial communities isolated from petroleum reservoirs included H2/ C02, lactate, formate, propionate, butyrate, and other fatty acids (up to C7), ethanol, methanol, glucose, peptone, benzoate, ketones, molasses, and crude oil [1,17]. Most pure cultures of sulfate-reducing bacteria isolated from petroleum reservoirs cannot grow on hydrocarbons. A range of these bacteria are able to grow autotrophically on H2/C02 (Desulfacinum infernum, Desulfacinum subterraneum, Desulfotomaculum sp, Desulfotomaculum kuznetsovii, Desulfomicrobium apsheronum, Desulfobacterium cetonicum) or in the presence of acetate or yeast extract as additional carbon sources. Organotrophic hyperthermophilic archaea Archaeoglobus fulgidus isolated from petroleum reservoirs of the North Sea and Alaska grow on complex substrates and oxidize lactate completely. Recently, A. fulgidus was shown to be capable of utilizing alkenes and related fatty acids with thiosulfate as an electron acceptor [18]. Sulfate, sulfite, and thiosulfate are the most common electron acceptors for sulfate-reducing bacteria; some organisms are also able to reduce elemental sulfur.
A search for anaerobic organisms decomposing hydrocarbons and petroleum is presently under way. Pure cultures of hydrocarbon-utilizing sulfate-reducing bacteria were initially isolated from terrestrial and marine environments by Widdel and coworkers [19,20]. Anaerobic biodegradation of n-alkanes is now a well-documented process that has been demonstrated under nitrate-reducing, sulfate-reducing and methanogenic conditions [21-23]. Most of the microorganisms were isolated from terrestrial environments. Mesophilic sulfidogenic enrichments growing on hexadecane were obtained from a carbonate collector of the Romashkinskoe reservoir (bed 302, Russia) [9]. A thermophilic sulfidogenic enrichment utilizing crude oil was also isolated from the White Tiger reservoir (Vietnam) [24]. A pure culture of the sulfate-reducing bacterium Desulfoglaeba alkanexedens utilizing C6-C12 n-alkanes was isolated from an oil reservoir in Oklahoma (United States) [16]. These works demonstrate abundance of sulfate-reducing bacteria capable of anaerobic hydrocarbon degradation in the reservoirs with temperatures from 18 to 80 °C. A range of special reviews was devoted to the genes governing hydrocarbon degradation by anaerobic bacteria [21,25]; this problem is, however, beyond the scope of the present study.
Rates of microbial processes in terrigenous petroleum reservoirs
In terrigenous petroleum reservoirs with sandstone collectors and low sulfate content in formation water, methanogenesis is the predominant process [26,27]. In sulfate-containing formation water both processes proceed simultaneously with different rates. The absence of sulfate limited sulfate reduction in the waters of Mykhpaiskoe and Samotlor oilfields [8,28]. Injection of sulfate-enriched water stimulated sulfidogenesis in the Binagady and Daqing oilfields, where SO42- and H2S were initially absent [6,10]. Addition of H2, acetate, or crude oil stimulated methane production in formation water samples from the Mykhpaiskoe (Russia) and Dagang oilfields (Figure 2) [8,29]. Data on extremely high rates of microbial processes in oilfields registered in the course of application of various MEOR technologies were not included In Table 1 [11,14]. Addition of H2/CO2 with acetate to the water samples of the Rinkon reservoir (California, United States) had a more pronounced effect on methanogenesis than addition of H2/CO2 alone [30,31]. These results demonstrate that, in spite of abundant petroleum organic matter, available utilizable sources of carbon and energy are scarce in petroleum reservoirs. The lowest rates of microbial processes were observed at heavy oil deposits (Chernoozerskoe and Yuzhno-Suncheleevskoe, Russia) with highly mineralized formation waters, which were exploited without water-flooding (Table 1).
Gieg and co-authors [31] estimated the rates of sulfate reduction in two high-temperature distinct oilfields on the North Slope of Alaska (designated Fields A and B). Rate measurements revealed that sulfate reduction was an important electron accepting process in Field A (6-807mmol S L-1 day-1), but of lesser consequence in Field B (0.1-10mmol S L-1 day-1). The signature metabolites of anaerobic hydrocarbon degradation were detected in produced water from the two oilfields offering evidence of in situ biodegradation activity. A methanogenic consortium obtained from Field B did showed a strong functional similarity with the high-temperature oilfield system and its 16Sr RNA gene clone library was similar to the sequences retrieved from other high-temperature reservoirs [28,30-35].
Possible growth substrates for microbial communities of petroleum reservoirs
Crude oil is the main substrate for microorganisms inhabiting the oilfields. Geochemical data indicate that petroleum biodegradation occurs in reservoirs with temperatures not exceeding 80 °C [36]. This is supported by microbiological investigation of petroleum reservoirs, analysis of the rates of biogenic processes, and research in microbial biology, which confirm that 80-85 °C is the upper temperature limit for petroleum biodegradation. Under subsurface conditions crude oil biodegradation is usually a slow process. Assuming that biodegradation occurs in the lowest 2% of the oil column, Head et al. [37] calculated hydrocarbon destruction fluxes at the base of oil columns to be of the order of 10-4kg hydrocarbons m-2 yr-1 for reservoirs at 40-70 °C.
The probable mechanisms of hydrocarbon degradation to methane in reservoirs were elucidated by Jones et al. [38] in laboratory experiments. In microcosms the hydrocarbon composition of degraded oil and generated gases was monitored, together with the carbon isotopic compositions of gas and oil samples taken at wellheads. Crude-oil hydrocarbon degradation under methanogenic conditions in the laboratory was found to mimic the characteristic sequential removal of compound classes seen in reservoir-degraded petroleum. The initial preferential removal of n-alkanes generated the amounts of methane close to stoichiometric ones, principally by hydrogenotrophic methanogenesis. This conclusion was supported by molecular studies of the methanogen community composition in oil-degrading microcosms. As much as 86-87% of archaeal 16S rRNA genes in clone libraries were close to the 16Sr RNA genes of hydrogenotrophic methanogens. Thus, both the highly enriched CO2 carbon isotopic signatures in heavily degraded oils and laboratory data suggest that methanogenic hydrocarbon degradation occurs predominantly via syntrophic oxidation of alkanes to acetate and hydrogen.
Davydova - Charakhchian et al. [39] were the first to show that acetate in high-temperature oilfield was decomposed by a binary syntrophic association with a lithoautotrophic methanogen Methanothermobacter thermoautotrophicus (Methanobacterium thermoautotrophicum=M. thermoalcaliphilum) as the terminal component. The acetate-oxidizing component of this association obtained from the Mykhpaiskoe high- temperature oilfield was not identified. Later Nilsen & Torsvik [40] observed methane formation in enrichment cultures with acetate at the temperatures of 70, 80, and 92 °C, but attempts to isolate acetate-utilizing methanogens were also unsuccessful. Indication of syntrophic acetate degradation as the main methanogenic pathway in high- temperature Dagang and Shengli oilfields (China) and in Yabase oilfield (Japan) was also obtained [23,33,41].
The process of methane production in formation waters of the Dagang high-temperature petroleum reservoir was registered by radioisotope methods with NaH14CO3 and 14CH3COONa and was in a range 0-1.595 and 0-14.73μg CH4 L-1 day-1, respectively (Table 1). However, pure cultures of thermophilic aceticlastic methanogens were not obtained. It was revealed that in this oilfield aceticlastic methanogenesis was probably not pronounced [11,33,42]. Acetate was decomposed to methane and CO2 by a syntrophic association including acetate-degrading bacteria of the Thermoanaerobacter-Caldanaerobacter group, which supplied hydrogen for the H2-utilizing methanogens of the genus Methanothermobacter. Shestakova and co-authors [42] for the first time isolated in pure cultures both components of syntrophic methanogenic associations and identified them as Thermoanaerobacter ethanolicus and Methanothermobacter thermoautotrophicus (and Methanothermobacter wolfeii). Experiments with labelled 2-14C acetate revealed that the binary culture combining T ethanolicus and M. thermoautotrophicus (or Methanothermobacter wolfeii) carried out the reaction of syntrophic acetate degradation to methane. The data obtained show that syntrophic associations may contribute significantly to methane production in high-temperature petroleum reservoirs. As a result of this study, an important ecological function of fermentative bacteria of the Thermoanaerobacter- Caldanaerobacter group was revealed, consisting in their ability to carry out syntrophic acetate oxidation to H2 and C02, which is then reduced to methane by hydrogenothrophic methanogens.
This work suggests important biogeochemical conclusions. Since methane formation in the presence of acetate may be carried out either by aceticlastic methanogens or by syntrophic associations, quantitative assessment of the rates of these processes requires experiments with labeled acetate both without antibiotic, as well as with a penicillin group antibiotic suppressing the growth of bacteria involved in syntrophic acetate oxidation. The difference between these values will indicate the rate of aceticlastic methanogenesis. Quantitative estimation of the rate of syntrophic acetate degradation to methane in petroleum reservoirs is presently not available in the literature.
Hydrogen of biogenic and abiogenic origin, volatile fatty acids (acetate and propionate) and crude oil are the substrates utilized by syntrophic methanogenic communities from petroleum reservoirs, containing methanogens and proton- reducing bacteria [23,38,40]. Methylated compounds have not yet been investigated as possible substrates for halophilic methanogens in formation water, although methanogenesis on methylamine was detected by the radioisotope method [43,44]. Thus, neither true halophilic, nor hyperthermophilic acetate- utilizing methanogens were found in petroleum reservoirs.
Pure cultures of mesophilic and thermophilic methanogens utilizing H2/CO2 have been isolated from petroleum reservoirs [1,27]. Acetate-utilizing methanogens have been isolated only from petroleum reservoirs with temperatures not exceeding 50°C. Aceticlastic methanogens growing at above 80 °C were not revealed in petroleum reservoirs [28]; they have not been described in the literature. High acetate content in formation waters may result from the rate of its formation as a result of petroleum biodegradation exceeding the rate of consumption. Available data suggest that molecular hydrogen is a major component in the chain of organic matter decomposition at the temperatures exceeding 70°C. Hydrogenotrophic methanogens, including M. thermoautotrophicus, M. thermoaggregans, M. thermolithotrophicus, Methanothermobacter tenebrarum, or Methanothermobacter crinale could be producers of methane under such conditions [22,23,33,39,40-42,45-46].
In the Bondyuzhskoe petroleum reservoir with a temperature 30-40 °C and high salinity of formation water, methane was formed mainly from labeled bicarbonate, rather than from acetate [43,44]. Methanogenesis was reported at formation water salinity from 15.8 to 274.5g/l; increase in salinity resulted in lower methanogenesis rates. Maximum methanogenesis rate of 4.95μg CH4 L-1 day-1 was found in the desalinized water of the near-bottom zone of injection well [44]. The contribution of the acetate methyl group to methane production was between 4 and 26%. Methane was also produced from labeled methylamine; however, the data on the presence of this substrate in the reservoir are not available. Hydrogenotrophic methanogens of the genus Methanobacterium isolated from the Bondyuzhskoe reservoir grew at salinity not exceeding 3% NaCl (M. bryantii, M. ivanovii, M. formicicum) or 8% NaCl (M. formicicum) [43]. Organotrophic methanogens Methanosarcina mazei and Methanococcoides euhalobius (=Methanohalophilus euhalobius) were most resistant to salt and grew at the total salinity of 100 u 140g/l, respectively [43,47].
In formation waters of sandstone oil-bearing collectors of the Kongdian bed of the Dagang high-temperature oilfield (China), sulfate was practically absent. Nevertheless both methanogenesis and sulfate reduction were registered as the terminal process of oil degradation (Table 1). In the course of application of the MEOR technology, based on injection of water-air mixture and nitrogen and phosphorous mineral salts, an increase of sulfate content in formation waters (from 0 to 178mg/l) was found [11]. Washing of sulfates from the oil- bearing rocks was observed earlier by Rozanova and co-authors [12]. We believe that FeS present in oil-bearing rocks could be oxidized by microorganisms in the near-bottom zone of injection wells where oxygen arrives dissolved in injection water and this process (es) resulted in production of sulfate which stimulated sulfate reduction. Ferric iron may act as an electron acceptor for iron-reducing bacteria of the genus Shewanella, which were isolated from the Dagang oilfield [48].
High numbers of cultivable sulfate-reducing or methanogenic microorganisms in formation waters do not necessarily correlate with the rates of microbial processes. This was confirmed by investigation of the formation fluid from production well 10941 of the Kongdian bed of the Dagang oilfield [11,49]. During the application of the MEOR technology, the activation process was completed and the concentrations of substrates required for growth of anaerobic microorganisms were low. Although methanogenesis rate was not registered by radioisotope technique, the number of cultivable methanogens remained high (104 cells•mL-1). The number of sulfate-reducing bacteria also reached 105 cells•mL-1, although in that period sulfate was absent in formation water and the registered rate of sulfate reduction decreased to zero.
Cultivable sulfate-reducing bacteria may occur in high numbers in formation waters containing no sulfates. It is known that in the presence of sulfate and other oxidized sulfur compounds sulfate-reducing bacteria are able to utilize a range of low-molecular-weight organic compounds (fatty acids, alcohols, lactate, acetate, and pyruvate). Some strains grow on more complex organic compounds, such as indole, phenol, catechol, saturated unbranched hydrocarbons, and alkyl benzenes [16,20,22]. A number of strains capable of reducing nitrate, Fe3+, U (VI), Se (VI), and Cr(VI) have been isolated. It is important that in the absence of chemical electron acceptors these bacteria are able to grow and to obtain energy from fermentation and interspecies electron transfer using methanogens as a biological acceptor of electrons. In this case, sulfate-reducing bacteria are possibly involved in the carbon cycle and transform hydrocarbons or products of oil degradation together with fermentative and methanogenic microorganisms.
These data especially require consideration when the results of molecular investigation of microbial phylogenetic diversity in petroleum fields are interpreted. Detection of the 16Sr RNA genes or specific functional genes in gene libraries does not confirm the relevant microbial geochemical activity in the reservoir. Molecular studies can offer insights into the organisms that may be active in the environment, but they underestimate the complexity and diversity of microbial interactions that are involved in petroleum biodegradation in oil fields.
Jiménez and co-authors [50] investigated in situ biotransformation of oil to methane in the Dagang oilfield using chemical fingerprinting, isotopic analyzes and molecular and biological methods. Geochemical data from oil, water and gas samples taken from the reservoir were consistent with in situ biogenic methane production coupled to the degradation of aliphatic and aromatic hydrocarbons. It was shown that autochthonous microbiota was capable of producing methane from 13C labeled n-hexadecane or 2-methylnaphthalene, and it was suggested that further methanogenesis could occur from the degradation of aromatic and polyaromatic fractions of the Dagang reservoir fluids.
Capacity of methanogenic and sulfate-reducing prokaryotes, responsible for the terminal stages of petroleum biodegradation, to separate (fractionate) the stable isotopes of carbon (ratios of 13C to 12C, 513C) and sulfur (ratios of 34S to 32S; δ34S), respectively is important for the understanding of their geochemical role. Preferential utilization of isotopically light compounds by these microorganisms and formation of methane and sulfide enriched with 12C and 32S, respectively were confirmed by numerous authors [24,27,38,49,51-53]. Together with direct measurement of the rates of biogenic processes by radiotracer techniques, comprehensive information on the changes in pH, redox conditions, stable isotope composition, and concentration of individual compounds in formation water, gas and oil (sulfate, carbonate, sulfide, methane, and carbon dioxide, as well as emergence of the products of petroleum degradation) may be used to confirm microbial geochemical activity.
Acetogenesis
To determine the fraction of total acetate production derived from reduction of carbon dioxide, NaH14CO3 was added to each water sample assuming that H2 was produced by microorganisms presented in the formation waters [43,44]. The production of acetate proceeded in waters from the Bondyuzhskoe oilfield with the salinity up to 237g L-1 at a mean rate of 17μg L-1 day 1, which is by two orders higher than the methanogenesis and sulfate reduction rates. Higher acetogenesis rates (37- 44μg L-1 day-1) were observed in the low salinity formation waters. Acetogenesis rate in waters from the high-temperature Mykhpaiskoe oilfield was in a range from 6 to 196|ig L-1 day-1 [44]. Since the rate of acetate destruction was 5 times higher than the rate of acetogenesis, other sources of acetate influx should exist.
Aerobic methane oxidation
Only few studies were devoted to aerobic methane oxidation in petroleum reservoirs. The rate of aerobic methane oxidation (14CH4) registered in formation water of the water-flooded petroleum reservoirs located in Tatarstan (Bondyuzhskoe, Romashkinskoe) was as high as 294 μL CH4 L-1 day-1 [54-59]. Methane-oxidizing bacteria were concentrated in the bottom zone of injection wells. Aerobic methane oxidation was not detected by radioisotope technique in the zone of production wells with highly mineralized water, probably because of the absence of dissolved oxygen, although viable cells of methane- oxidizing bacteria were present in formation water.
Assimilation of 1-14C-octadecane by microorganisms of the oilfield
*Backflushed water from injection well 1098, sample 8 m3. Nd -not determined.
Assimilation of saturated hydrocarbons by the microbial community of the Dagang high-temperature oil field, which has been exploited with water-flooding over 30 years, was studied using labeled 1-14C-octadecane. For analysis, formation water samples from the same horizon and similar in chemical composition were used, as well as injected water. The main difference was in the presence of small amounts of dissolved oxygen in injected water (1.6-2.8mg O2/l water) and in the fluid from the near-bottom zone of the injection well (0.4mg O2/l water), where oxygen arrived with injection water. Potential activity of hydrocarbon-oxidizing microorganisms in different zones of the water-flooded oil field was determined by incorporation of the labeled 1-14C-octadecane into microbial biomass. Formation water samples were sealed, avoiding air bubbles, injected with 1-14C-octadecane, and incubated for 24h at 60 °C. The samples were then fixed with saturated KOH and filtered through membrane filters (0.22μm). Oil was removed from the filters with chloroform and toluene, and dried filters were immersed in the scintillation liquid with β-phenylethylamine and methanol (3:1:1). Radioactivity was measured on a 1219 Rackbeta scintillation counter (LKB, Wallac, Turku, Finland). The highest incorporation of radioactive carbon from 1-14C-octadecane into the biomass (counts/min) observed in injected water sample was accepted as 100% (Table 2).
Radioactivity of the biomass from the liquid collected from the near-bottom zone of the injection well was 36.1%, and in water from production wells was in the range of 0.01-0.39% from the value for injection water. Incorporation of carbon from 1-14C-octadecane into the biomass of the nearbottom zone microorganisms may indicate active hydrocarbon oxidation in the oil field zone supplied with dissolved oxygen, biogenic elements, and enhance water and mass exchange. 14C incorporation into microbial biomass from producing wells may indicate the presence of microorganisms capable of utilizing this hydrocarbon under anoxic conditions. Thus, potential rate of biodegradation of oil n-alkanes in a water-flooded oilfield was much higher in the oxidized zone close to injection wells than in the anoxic zone, where dissolved oxygen was absent.
Conclusion
Understanding of diversity and functioning of oil field microbial communities will lead to deeper and more accurate prediction of the processes in this ecosystem and possibly to intentional control of these processes. Development of modern biotechnologies for enhanced oil recovery, suppression of sulfidogenic populations and corrosion of metal equipment, improvement of oil quality, and conversion of residual oil to methane require comprehensive investigation of the microorganisms in petroleum reservoirs. The application of radioisotope approach, together with geochemical analysis of the environment, methods of analysis of stable isotope composition, cultivation methods and molecular studies of microbial communities are necessary tools for understanding microbial processes in petroleum reservoirs.
Acknowledgment
This work was supported by the Russian Science Foundation (grant no. 16-14-00028).
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