Research Article
The Importance of Immunohistochemical Staining with DNAJB9 for the Diagnosis of Fibrillar Glomerulonephritis
David Campos Wanderley1,2* and Stanley de Almeida Araújo1,2
1Institute of Nephropathology, Belo Horizonte, Minas Gerais, Brazil
2Electronic Microscopy Center, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil
Received Date: April 20, 2020Published Date: April 23, 2020
Corresponding author: David Campos Wanderley. Avenida Bernardo Monteiro, 971. CEP: 30.150-283. Belo Horizonte, Minas Gerais, Brasil
How
to cite this article David
C W, Stanley d A A. The Importance of Immunohistochemical Staining
with DNAJB9 for the Diagnosis of Fibrillar Glomerulonephritis. JOJ
Urology & Nephrology, 2020; 7(2): 555710.
DOI: 10.19080/JOJUN.2020.07.555710
Abstract
Fibrillary Glomerulonephritis (FGN) is a rare glomerulopathy, corresponding to less than 1% of all renal biopsies performed. The pathogenesis mechanisms of FGN are still not completely understood and most cases are considered idiopathic. The clinical presentation is characterized by proteinuria, hematuria, arterial hypertension and renal failure. The evaluation of FGN is a diagnostic challenge in the practice of the pathologist, because there are no morphological characteristics with highly specific or pathognomonic lesion. The use of a sensitive and specific biomarker can make an important contribution to renal pathology and the differential diagnosis of diseases with fibrillar deposits. In this contexto, DNAJB9 can be investigated by immunohistochemical technique and is useful for the diagnostic definition of FGN.
Keywords: DNAJB9, Biomarker, Immunohistochemistry, Kidney, Fibrillary GN
Abbreviations: FGN: Fibrillary Glomerulonephritis; LM: light microscopy; IF: immunofluorescence; EM: electron microscopy
Introduction
What is Fibrillary Glomerulonephritis?
Fibrillary Glomerulonephritis (FGN) is a rare glomerulopathy, corresponding to less than 1% of all renal biopsies performed [1]. This entity was first described by Rosenmann and Eliakim in 1977, who reported a patient with nephrotic syndrome who identified a glomerular deposition of amorphous material, which resembled amyloid by light microscopy, but their fibrils shorter than amyloid fibrils in ultrastructural analysis [2]. However, it was only in 1983 that FGN was defined as a distinct glomerular disease [3]. The pathogenesis mechanisms of FGN are still not completely understood and most cases are considered idiopathic [1,4-7]. Approximately one third of the cases, an etiological association is found with malignant neoplasms, dysproteinemia, hepatitis C and autoimmune diseases [7,8]. The clinical presentation is characterized by proteinuria (100%), hematuria (~ 50%), systemic arterial hypertension (~70%) and renal failure [5-7]. Renal survival is poor, with almost 50% progression to end-stage renal disease within 4 years; however, with good patient survival, being 80% at 4 years of follow-up [7]. In addition, because there is no effective therapy, a recurrence rate after a kidney allografts variable between 35-50% [9].
How is a Fibrillary Glomerulonephritis Diagnosed?
Until recently, the
diagnosis of FGN was only possible performing light microscopy (LM),
immunofluorescence (IF) and electron microscopy (EM). The EM evidence
accumulation od fibrils randomly
oriented, straight and nonbranching,
with measurements between 10 and 30 nm in thickness. The is
proeminente in memesangium and/or along the glomerular basement
membranes [1,5,7,10]. IF, in most cases, shows smudgy glomerular
staining for IgG, C3 and light chains (kappa and lambda). The IgG4
subtype is the most prevalent, presenting restriction in most cases
(80%); moreover, light chains usually exhibit the same intensity of
IF stain [1,5,6,11,12]. The LM can exhibit several morphological
patterns, but in most cases it shows mesangial proliferation with or
without duplication of the glomerular basement membranes
(membranoproliferative glomerulonaphritis), with crescentic lesions
identified in approximately 25% of cases [1,5,6,7,13].
Congo red research is important to distinguish FGN from amyloidosis. Deposits in FGN are negative for Congo red, with no birefringence on the green apple under polarized light in the vast majority of cases. However, although infrequent, there is a study that shows Congo red positivity in some patients with FGN [14]. The evaluation of FGN is a diagnostic challenge in the practice of the pathologist, because the main diagnostic methods (ML, IF, ME) have limitations. There are no morphological characteristics with highly specific or pathognomonic lesion. Added to this is the fact that such characteristics can also be seen in several other glomerulopathies, such as: Amyloidosis, IgA Nephropathy, Diabetic Glomerulopathy, Immunotactoid Glomerulopathy, Fibronectin Glomerulopathy, Collagen-fibrotic Glomerulopathy, among other deposit glomerulopathies. In addition, access to EM is costly and restricted to a minority of cases, especially in developing countries. Thus, it is clear that FGN is an underdiagnosed disease and that the use of a sensitive and specific biomarker can make an important contribution to renal pathology and the differential diagnosis of diseases with fibrillar deposits. In this context, a new proteomic biomarker, DNAJB9, has been discovered, which can be investigated by immunohistochemical technique and is useful for the diagnostic definition of FGN.
What is DNAJB9 and what is its importance in this context?
DNAJB9 is a protein that contains 223-amino acids and is a member of the DNAJ family. DNAJ proteins participate of several fundamental roles, such as regulating ATPases activity, stressing the endoplasmic reticulum, inflammatory mediators actions, differentiating B lymphocytes and producing antibodies, in addition they have protective function against cell death and protects hematopoietic stems cells during stress [15-21]. In the mass spectrometry study, this protein deposition was identified in the glomeruli of all patients diagnosed with FGN and this accumulation was not found in others glomerulopathies [4,22,23]. When deposited in the FGN, curiously, no structural protein change was identified [22]. The presence of DNAJB9 can be assessed by immunohistochemistry, which is available in some laboratories specialized in kidney pathology. The first studies with this marker have shown high sensitivity (98-100%) and specificity (99-100%) in the diagnosis of FGN [4,22,23].
Conclusion
The research of the DNAJB9 protein by immunohistochemical technique has shown high accuracy for the diagnosis of FGN, enabling the diagnostic differentiation with several other glomerulopathies, especially those that exhibit fibrillar deposits. Its discovery and applicability is promising and will allow greater diagnostic precision, avoiding errors and underdiagnosis of FGN.
Conflict of Interest
The authors declare that they have no conflict of interest
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