Submission: April 10, 2018; Published: May 01, 2018
*Corresponding author: Nguyen Thi Trang, Hanoi Medical University, UK, Email: email@example.com
How to cite this article: Nguyen Thi Trang, Nguyen Minh Thu, Nguyen Thi Quynh. Application of Realtime-PCR for Detection of 11 Urogenital Infections. JOJ uro & nephron. 2018; 5(3): 555665. DOI: 10.19080/JOJUN.2018.05.555665
Sexually transmitted diseases (STD) are a group of diseases that are common disease. They are not only affecting health, especially reproductive health but also affecting the socio - economy. Grace of the development of science and technology, now we have many modern methods for quick and accurate diagnosis STDs. A cross-sectional descriptive study, 300 men were consider genital infections. The samples were tested by Realtime PCR for identifing 11 microorganism. As a result, the rate infected microorganism is 72.9%. The most common infection was Gardnerella vaginalis (45.7%). The rates of mononucleosis and multiple infections were 49% and 51%. Gardnerella vaginalis is the most common cause single infection and co-infection. Patients with age from 20 to 29 is the biggest group of men who are consider suffer from STD (42.7%) and also is the biggest group infections STD (43.9%).
Urogenital infections are a worldwide shared problem that represent the most common reason for a woman to decide to visit to gynaecologist or urologist . A urinary tract infection (UTI) is an infection that affects part of the urinary tract. When it affects the lower urinary tract, it is known as a simple cystitis (a bladder infection) and when it affects the upper urinary tract, it is known as pyelonephritis (a kidney infection). The indigenous microbiota plays an important role in protecting the host from colonization of invading pathogens . The most common pathogens are Chlamydia trachomatis, Neisseria gonorrhoeae, Gardnerella vaginalis....
The positive result is 72.9%, which is higher than that of WHO
 . This is because the prevalence of patients is higher than that of the population. In the group of patients with positive results, the most common age is also from 20 to 29 years. This age group is older than that of N Fournet et al. , due to differences in sexual habits between Vietnam and other countries  (Figure 2).
Over the study period, the most common species is G. vaginalis (45.7%). But other study report a difference in results (C. trachomatis and N. gonorrhoeae) . These differences could be explained by changes in study population characteristics over time and space. Another reason is the difference in test method
 (Figure 3,4).
The percentage between two groups was similar (49% - 50%). Patients with more microorganisms, the lower the percentage. At maximum, one patient had 7 microorganisms. Not only in the group of mono-infected but also in the group of multi-infected patients, the most common microorganisms is G. vaginalis. Mycoplasmas do not often appear alone but in combination with other microorganisms (Figure 5) (Table 1).