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Evaluation of Negative Energy Balance in
Dairy Cows in Qom Province, and its
Relationship with Periparturient Diseases
Mohammad Reza Mohebbi1*, Samad Lotfollahzadeh1 and Majid Mohammad Sadegh2
1Faculty of Veterinary Medicine, Department of Internal Medicine, University of Tehran, Iran
2Assistant professor of theriogenology, Islamic Azad University, Iran
Submission: February 01, 2019;Published: February 21, 2019
*Corresponding author: Sevidzem Silas Mohammad Reza Mohebbi, Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Qareeb Street, Azadi Avenue, Postal Code:1419963111, P.O.Box : 14155-6453, Tehran, Iran
How to cite this article: Md Reza M, Samad L, Majid Md S. Evaluation of Negative Energy Balance in Dairy Cows in Qom Province, and its
Relationship with Periparturient Diseases. Dairy and Vet Sci J. 2019; 10(2): 555783. DOI: 10.19080/JDVS.2019.10.555783
Objective: The aim of this study was to evaluate the correlation between negative energy balance (NEB) and periparturient diseases and to introduce blood BHBA and glucose values as instruments to detect cows with NEB to prevent its harmful effects on immunity and the incidence of periparturient.
Material and Methods: 207 fresh Holstein cows were being investigated over the 2-4 weeks after parturition. BHBA and glucose concentrations were determined, using portable strip test by Optimum Xceed® (Ireland MediSense®Optium), data were evaluated by statistical programs and the results were recorded.
Results: The incidence of NEB in central Iran (with threshold of 1 mmol/l for serum BHBA values) was 16.3 % that is in range of other reports. Although the incidence of preparturient diseases other than RP was lower than other studies. Serum BHBA values in cows with periparturient diseases were significantly higher than cows with no periparturient diseases. There was no significant correlation between milk production and the values of serum BHBA (P<0/05). Days open and parity were significantly higher in cows with NEB in comparison with cows with no NEB. There was no significant correlation between serum glucose values and periparturient diseases, parity, milk production, and days open (p>0/05)
Keywords: Negative energy balance; Glucose; BHBA; Periparturient diseases
Negative energy balance (NEB) is a circumstance occurring in high milk producing fresh cows. It defined as an imbalance between dry matter intake and production needs . After parturition, cows face a sharp increase in production and loss of appetite due to calving stress and high levels of diet change . NEB can be detected by relatively high concentrations of the ketone bodies acetoacetate, β-hydroxybutyrate (BHBA) and acetone, and a simultaneous low concentration of glucose in the blood [2-4]. The cows decline to mobilization of adipose reserves, and they often lose 60% or more of their body fat in the first weeks of calving [5,6]. Failure of hepatic gluconeogenesis to compensate energy requirements for milk production and body needs, may be one cause of NEB .
The effects of NEB on reproductive system was demonstrated . The period of NAB is accompanied with immunosuppression, periparturient diseases and increased times to first ovulation and first service . The objective of this study was to evaluate the correlation between NEB and periparturient acci
dents in dairy cows of Qum province and subsequently to assay the reliability of blood BHBA and glucose values to prevent NEB disorders.
Two hundred-seven fresh Holstein cows were being investigated after parturition in dairy farms of central Iran.
They were selected randomly based on their calving date during September 2010 to May 2011. They were milked twice daily and milk yield was recorded once a week. All information such as parity, date of pregnancy, service to pregnancy ratio, last days open, milk production, etc. were recorded in individual forms for each cow.
Blood samples were collected from all cows in 1-2 weeks after parturition, at least 4 hours after feeding for minimizing the effect of feeding on the results. BHBA and glucose concentrations
were determined on-farm, using portable strip test by
Optium Xceed® (Ireland MediSense®Optium), according to the
instruction of the producing company. Sensitivity and specificity
of the Optium Xceed® has been evaluated 100 % in one study
, 90% and 98 % respectively in another study .
In this study, SPSS software was used for statistical analysis
of the data. One Sample Kolmograv-Smimov test is used to determine
the normal distribution of the BHBA and glucose values
of blood serum. Kruskal-Wallis and One-way ANOVA tests were
used to evaluate the difference between BHBA and glucose in
various groups of sick and healthy cows, respectively. The Chisquare
test was used to evaluate the distribution of the cows in
two groups of normal and high BHBA and glucose levels of the
blood samples. Pearson test was also used to evaluate correlation
between days open, parity and milk production with BHBA
and glucose levels of the blood samples. The two tails level of
statistical significance was present at p≤ 0.05.
Averages of milk production, parity, weight of calves at
berth, days open, BHBA, and glucose values of blood serum were
10487±1090, 2.38±0.8, 40.38±1.04 kg, 191.149.08, 0.75±0.12
mmol/l, and 63.96±4.17 mg/dl, respectively. Averages of milk
production, parity, BHBA value, and glucose value in cows with
periparturient diseases were as followed: 10458±382.84 kg,
3.37±0.49, 1.44±0.42 mmol/l, and 56.26±10.34. Averages of
the above parameters in healthy cows were 10860±1477.84,
2.13±0.17, 0.56±0.11 mmol/l, and 68.31±14.41 mg/dl, respectively.
Statistical tests indicated that serum BHBA values in cows
with periparturient diseases were significantly higher than cows
with no periparturient diseases. Table 1 shows the average values
of BHBA and glucose in healthy cows and cows with periparturient
diseases. Statistical tests indicated the significant
difference between serum values of BHBA in healthy cows and
cows with periparturent diseases (p=0.01), but the difference
between serum values of glucose in healthy cows and cows with
periparturient diseases were insignificant (p>0.05).
In this study threshold of 1 mmol/l for serum BHBA is indicated
as a cut point of NEB. Healthy cows with no periparturent
diseases and cows with periparturent diseases were compered
based on the standard threshold of BHBA (1mmol/l) (24 the) and the K-square test showed that the cows with periparturient
diseases significantly have the BHBA values higher than 1
mmol/l (p<0.05) (Table 2). Totally 57.17 % of cows with NEB,
involved with periparturent diseases compared with 6.8 % of
cows with no NEB. Comparison between cows with no NEB and
cows with NEB, about days open is showed in Table 3 and cows
with NEB significantly had the higher days open (P<0.5). Also,
the incidence of NEB was compared with number of parity and
the group with NEB had significantly more parity (3.38 parity)
than group with no NEB (2.19 parity) (Table 4).
Duffield introduced two tests to investigate NEB, pre-calving
NEFA and post-calving BHBA blood values . In this study,
measurement of BHBA and glucose blood values in fresh cows
is used to evaluate NEB. Kocako  showed the rate of periparturent
diseases in fresh cows as: milk fever 7.2 %, DA 3.3 %,
ketosis 3.7 %, and RP 9 % . In the current study, incidence of
periparturent diseases was recorded as: milk fever1.4 %, DA 1.4
%, clinical ketosis 1 %, RP 9.5 %, septic metritis 1 %, and mastitis
1.4 %. All diseases other than RP had the lower incidence in comparison
with other studies; however, in one study Tai-Young 
recorded the incidence of RP 14 % which is higher than current
study. The prevalence of subclinical ketosis was reported in wide
ranges. Prevalence for increase in BHBA in fresh cows has ranged
from 8.9 to 34 % in different studies. In this study, 16.3% of sampled
fresh cows had the BHBA higher than 1mmol/l (Table 2).
In most studies, NEB has most correlation with DA . In
current study there was a significant relationship between NEB
and the incidence of DA, RP, and metritis. It has been demonstrated
that the severity and duration of NEB have the positive correlation
with the time of first ovulation after calving [16,17]. And,
in another study animals with BHBA concentrations >or=10mg/
dL had a 13% decrease in risk of pregnancy . In this study,
the correlation between days open and serum BHBA values was
calculated. Statistical analysis showed that mean days open in
group of cows with BHBA >1 mmol/l is 131.5 days and in a group
of cows with BHBA <1 mmol/l is 93/19 mmol/l (p<0.05). In two
study greater serum BHBA post-calving were associated with
less milk yield, greater milk fat percentage, and less milk protein
percentage [18,19]. But Kaupe  indicated that there is
a significant positive relationship between BHBA concentration
and milk production while the cows with increased BHBA significantly
have the higher milk production that can be result in being
in the higher risk for ketosis in this group .
There was no significant correlation between milk production
and the values of serum BHBA (P<0/05). Parity 1 cows mobilized
fewer fatty resources than parity 2 and 3 cows . But
in another study, there was no meaningful correlation between
parity and ketosis . In current study mean parity of cows with
BHBA>1 mmol/l was 3.38 and of cows with BHBA<1 mmol/l was
2.19 (p<0.05). Several studies reported that concentrations of
plasma glucose and insulin in cows with fatty liver disease were
not lower [22,23]. In another study IGF-I (PZ0.001) and glucose
(PZ0.001) concentrations, were lower in Sever NEB in comparison
with Mild NEB cows [24-26]. In this study mean glucose
concentration in cows with BHBA>1 and cows with BHBA<1 was
42.5±4.72 mg/dl and 69.46±3.76 mg/dl respectively (p<0.05).
But There was no significant correlation between serum glucose
values and periparturent diseases, parity, milk production, and
days open (p>0.05).
Based on the findings of this study, measurement of blood
BHBA and glucose values are reliable to detect NEB and prevention
of periparturient diseases with more emphasis on BHBA.
The authors declare that this study is done in the limited geographic
area and in limited cow sample size and it needs to be
done in larger population considering other involving factors
such as dry mater intake before and after parturition.