Opinion

Tissue Culture of Moringa oleifera Lam

Totik Sri Mariani* and Heni Hanurati

Biosciences and Biotechnology Research Center, Bandung Institute of Technology, Indonesia

Submission: November 15, 2021;Published: December 02, 2021

*Corresponding author: Totik Sri Mariani, Biosciences and Biotechnology Research Center, Bandung Institute of Technology, Indonesia

How to cite this article: Totik Sri M, Heni H. Tissue Culture of Moringa oleifera Lam. JOJ Hortic Arboric. 2021; 3(2): 555610. D. DOI 10.19080/JOJHA.2021.03.555610.

Opinion

Moringa oleifera is a miracle tree. It contains vitamin, mineral, lipid, amino acid, quercetin and moringin. It is also known as an herbal medicine. It could cure many diseases, such as diabetic, cancer, hypertension, inflammation, etc. Since many people harvested this plant from nature, the existence of Moringa is decreasing. Therefore, it is important to propagate this plant by tissue culture method (micropropagation).

We have been propagating the Moringa in plant tissue culture laboratory. For micropropagation, the explant used was nodes. The nodes were grown from seeds. Figure 1 shows the seeds of Moringa oleifera. The explant could grow into shoot and root. Murashige and Skoog medium with Gamborg vitamin was used for germinating the seeds. Thereafter, the nodes were isolated and grown in the shoot initiation medium (SIM). The SIM was the same as germination medium with the addition of 2 ppm BAP (Benzyl Amino Purine). Thereafter, the growing shoots were multiplicated in shoot multiplicated medium (SMM). The SMM was the same as SIM. As for rooting, 1 ppm of IBA (Indole Butyric Acid) was supplemented in rooting medium (RM). After root was growing, the plantlets were established. Figure 2 shows the plantlets of Moringa oleifera. From this research, we can acclimatize Moringa plantlets and transfer the plants to the field. Hopefully, the Moringa plants could survive and propagate by themselves in nature. In my opinion, tissue culture of Moringa oleifera is easy and will give a benefit for Moringa plantation.