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Phenolic Substances and Biological Activities
of Verbana officinalis L.: A Mini-Review
Hajer Riguene1, Ghayth Rigane1,2* and Ridha Ben Salem1
1Organic Chemistry Laboratory LR17ES08, Sciences of Sfax Faculty, Chemistry Department, University of Sfax, Tunisia
2Faculty of Sciences and Technology of Sidi Bouzid, Department of Physics & Chemistry, University of Kairouan, Sidi Bouzid, Tunisia
Submission: January 15, 2019; Published: February 05, 2020
*Corresponding author: Ghayth Rigane, Organic Chemistry Laboratory LR17ES08, Sciences Faculty of Sfax, University of Sfax, Tunisia.
How to cite this article: Hajer R, Gayth R, Ridha B S. Phenolic Substances and Biological Activities of Verbana Officinalis L.: A Mini-Review. Organic & Medicinal Chem IJ. 2020; 9(2): 555758. DOI: 10.19080/OMCIJ.2019.09.555758
The published data related to the identification of the major phenolic compounds as well as its biological activities present in Verbana officinalis L. This plant has received a great interest in the worldwide for its diuretic, expectorant and anti-rheumatic, anti-inflammatory, antifungal, antibacterial, analgesic and antioxidant activities.
Verbena officinalis or commonly referred as vervain belongs to the verbenaceae family (Table 1) which can be found in West Asia, North Africa and throughout Europe . It is listed in the Chinese Pharmacopoeia and the British Herbal Pharmacopoeia . Verbana is a medicinally used herb, where most people still depend on the folk medicine, despite the great progress in all opathic medicines, particularly to all eviateanxiety, insomnia, depression . Verbena officinalis has been widely used for amenorrhea, insufficient lactation (lactogogue plant), assisting contractions duringlabor, promoting wound healing and for disorders of menstruation .
Verbena officinalis is a perennial herb. It is erect, 25–100 cm tall and branched above. Its leaves are 3.5–8 cm long and 1.5–3.5 cm broad. It has pale pink or purplish color flowers about 4 mm across  (Figures 1 & 2).
The main class of compounds of these plants were phenylpropanoids,
being verbascoside the most abundant in all the preparations
up to 97 % of the total phenylpropanoids. In addition, iridoids,
has hastato side and verbenalin together with flavonoids,
mono- and di-glucuronidic derivatives of luteolin and apigenin
were found  (Table 2).
Several analytical techniques were used in order to identify
and quantify the phenolic composition of Verbena officinalis,
these techniques included high-performance liquid chromatography-
mass spectrometry (LC-MS) [6,7], high-performance liquid
chromatography-mass spectrometry/mass spectrometry LC-MS/
MS , ultra-high performance liquid chromatography diode array
detector UHPLC-DAD , Micellar electro kinetic chromatography
MECC  and High-performance liquid chromatography
with diode-array detection HPLC-DAD  (Table 3).
The famous properties of vervain herb are sedative, antispasmodic
and diaphoretic . Verbena officinalis L. has been traditionally
used as nervine tonic, antidepressant, and anticonvulsant;
prescribed in liver and gall bladder complaints (spasm of the
bladder and strangury), nervous and menstrual disorders; also,
for bronchitis, asthma and febrile affections .
In addition to that, Verbana officinalis can be utilized to treat
enteritis, acute dysentery, depression and amenorrhea . The
scavenging activity against DPPH (1,1diphenil-2-picrylhydrazyl)
radical and the antifungal effect against chloroform, ethylacetate
and 50% methanolic extracts of Verbena officinalis leaves were
investigated. The activity of different fractions of 50% methanolic
extract and some isolated compounds were also investigated. The results suggest that 50% methanolic extract and caffeoyl derivatives
could potentially be considered as excellent and readily available
sources of natural antifungal and antioxidant compounds .
The antimicrobial potential of verbana herb leaves, and roots
was evaluated against 24 strains of Gram-positive and Gram-negative
bacteria by Dildar . Ethanolic extracts of stems, leaves, and
roots of Verbena officinalis and their fractions in various solvents
were assessed. The stems proved to be most potent against all the
strains. Its activity against Staphylococcus aureus and Pseudomonas
aeruginos a was higher than the antibiotic Amoxicillin.
The leaves also showed considerable activity against Pseudomonas
aeruginosa, Citrobacter freundii, and Staphylococcus aureus.
The roots turned out to be highly effective against Bacillus
subtilis, Staphylococcus aureus, and Pseudomonas aeruginosa.
The study confirmed the efficacy of Verbena officinalis against infectious
diseases. While all the three parts of the plant were active
against the test micro-organisms, stems were most powerful. The
plant has great potential to provide exploitable leads for new antimicrobial
The biological activities of Verbana officinalis L., including antioxidant,
anti-microbial, anti-inflammatory and anti-cancer, were
due to the presence of bio-active compounds in the leaves such as:
Verbenalin, Hastatoside, Verbascoside, Luteolin-7-O-β-D-diglucuronide,
Encalada MA, Rehecho S, Ansorena D, Astiasaran I, Cavero RY, et al. (2015) Antiproliferative effect of phenylethanoid glycosides from Verbena officinalis L. on Colon Cancer Cell Lines. LWT - Food Sci Technol 63: 1016-1022.