Research Project of Anticancer Compounds Contain Amino acids, Vitamins and Flavonoids
Department of Medicinal Chemistry, India
Submission: September 10, 2017; Published: September 19, 2017
*Corresponding author: Mohammad Aldajani, India; Tel: 248-797-0538; Email: m_[email protected]
How to cite this article: Mohammad A. Research Project of Anticancer Compounds Contain Amino acids, Vitamins and Flavonoids. Organic & Medicinal Chem IJ. 2017; 3(5): 555621. DOI: 10.19080/OMCIJ.2017.03.555621.
Synthesized the following anti-cancer agents which gave positive tests with Topoisomerase kits. Studied these compounds further by using
1. HT-29, (Human Colon cancer cells)
2. CaOV (Ovary cancer cells)
3. T-47D (for ER-Positive breast cancer cells) and MDA - MB-231(for ER- negative Breast cells cancer).
Chrysin was used as a positive control for this study. All of them showed different Selectivity's with different concentrations.
a) Tested some of these compounds in Karmanos Cancer Center in Michigan against 1- human pancreatic cancer (BxPc-3) 2- Pancreas protein 1 (PANC1).
b) Examined the Toxin for these compounds by injecting the mice two times with 70 mg/kg intravenously and none of the mice died.
c) The results clearly show that these compounds are very promising and selective candidates as anti-cancer agents. All these compounds can dissolve in water
d) Next step is to develop new compounds based on experience as designing compounds using Protein-Ligand docking which will have better selectivity and activity.
The same compounds are re synthesize in Kamran's Cancer Center in Michigan, And tested against human pancreatic cancer (BxPc-3) and pancreas protein 1 (PANC1). Examined the Toxin for these compounds by injecting the mice two times with 70 mg/kg intravenously and none of the mice died (Figure 1) & (Figure 2).
a) The results clearly show that these compounds (31, PFC) are very promising for selected candidates as anti cancer agents.
b) These compounds kill more than 50% of the cancer cells at low concentrations 50|i/L.
c) Study found that the compounds was not toxic at 70mg/ Kg and mice did not die at this concentration when injected tow times.
a) Preliminary screening showed only PAAZNA, PSZNA and chrysin have cytotoxicity effects on T-47D.
b) Compounds PAAZNA and PSZNA emerged as the most active compound of the series in T-47D cells.
c) Particularly potent against T-47D cells when compared to chrysin.
d) Remaining compounds examined were not showing any cytotoxic effect on the cell line (Figure 5).
I. Preliminary screening showed only PGMOA, PSZNA and Chrysin have Cytotoxicity effects on MDA-MB-231.
II. The term half maximal effective concentration (EC50) refers to the concentration of a drug, antibody or toxicant which induces a response halfway between the baseline and maximum after a specified exposure time. It is commonly used as a measure of drug's potency
Trypan blue assay (viability test) dead cells are shown as blue color under a microscope but live cells are excluded from the stainin, LDH (lactate dehydrogenase) assay Determine the soluble cytosolic enzyme that is released into the culture medium following cell death resulting from either apoptosis or necrosis can be used as an indicator of cell membrane integrity (Figure 8).