Our experiment grafted Mouse Embryonic Stem Cell (mESC) to influence behavioral deficiency in rodent animal models of clip compressive surgery inducing Spinal Cord Injury (SCI) of central nervous system. Our research proved the effect of grafted stem cells to the injured spinal cord region, focusing the application of mouse embryonic stem cells for regeneration of spinal cord nervous injury. Therefore, our research suggests manifest results that implantation of mouse embryonic stem cell could show behavioral improvement after severe spinal cord damage.
It has been reported that administration of various stem cells applied into an injury-tissue area, resulting from a clip compression technique of the thoracic spinal cord region. These researches had suggested promotion of motor & behavioral function in injured animal models, as tested by the Basso, Beattie, Bresnahan motor rating scale method . Various trial of cell transplant has been showed to induce functional enhancement after severe spinal cord damage. Several studies evaluate lesser the progress of additional damage, minimizing the injured-inhibitory circumstance of the infarction region, changing damaged tissue with transplanted cells, rebuilding Schwann cells and axonal regeneration, and stimulating specific growth factor and intrinsic progenitor cells .
Previous researches in rodent models occurring spinal cord damage have revealed that stem cell transplants survived well in the damaged spinal cord region, filled the cavitation region and found several neuronal cell types. Other studies showed that cell transplant improves motor functional recovery and induces the plasticity of related motor neurons.
The clip compressive surgery used to induce spinal cord injury was a specific vascular aneurysm surgery clip, which has constantly been applied to induce neurologically spinal cord damage in animal models . The specific spinal cord site was completely pressed for 60 sec dorsoventrally.
Grafted cells survived to fill the injured cavity after transplantation of mESC. The spinal cord was stained for GFAP (green). mESC (yellow) labeled with a fluorescent dye migrated from their injection site to injured areas. Immunohistochemistry showed intensive glial reaction by GFAP-stained cells in the injured spinal cords (Figure 1). The transplanted mESC migrated into the injured sites and integrated into the scar areas. It is suggested that infiltrated mESC may produce trophic factors in order to reduce the cavities and improve behavioral function. However, transplanted mESC did not express GFAP, a astroglial marker.
Our research has demonstrated that the result of intracellular
implantation in the damaged animal spinal cord site is
proved to an effective & significant enhancement in the BBB scale
scores. Functional locomotive movement of the implanted group
animals could be proved by a greater survival of regenerative
axonal formation in the injured site. Previous studies strongly
suggested that implanted cell survival resulted by significant
reduction of neuronal cavitation tissue may facilitate motor and
functional recovery . mESC-transplantation group for at least
five weeks post-transplant showed partially filling the cavities
and connecting into the intrinsic spinal cord and resulted in
reduction of a large amount cavity formation. It has been reported
that reduction of cavity formation after spinal cord injury has
been also experimented by implantation of various stem cells
including marrow stromal cells [3,5] and Neural Progenitor Cells