Totik Sri Mariani

doi:10.19080/JOJHA.2024.04.555643

Research Article

Potential of Shoots from “Katak” For Shoot Apical Meristem Culture on Amorphophallus Konjac

Totik Sri Mariani¹, Ahmad Sjahrian S¹ and Tet Fatt C²

¹Bandung Institute of Technology, Bandung, Indonesia

²Tet Fatt Chia Impact Capital, Singapore

Submission: June 25, 2024; Published: July 04, 2024

*Corresponding author: Totik Sri Mariani, Bandung Institute of Technology, Bandung, Indonesia

How to cite this article: Totik Sri Mariani, Ahmad Sjahrian S, Tet Fatt C. Potential of Shoots from “Katak” For Shoot Apical Meristem Culture on Amorphophallus Konjac. JOJ Hortic Arboric. 2024; 4(4): 555643. DOI: 10.19080/JOJHA.2024.04.555643.

Keywords: Amorphophallus Konjac; Katak; Mass clonal propagation; TDZ solution; Shoot apical meristem

Opinion

Amorphophallus konjac is an herbaceous evergreen perennial plant with edible corms that contain two important reserve polysaccharides, starch and glucomannan [1]. It has many industries derived from polysaccharides of A. konjac. Therefore, we have to prepare the plantation of A. konjac by means of shoot apical meristem culture.

“Katak” (bulbil) of A. konjac is brown nodule that growing on the leaf of A. konjac so we can call it bulbil of leaf [2]. Figure 1 shows “katak” (bulbil) of A. konjac after detaching of leaf. With BAP (Benzyl Amino Purine) and TDZ (Thidiazuron), the “katak” will produce hundreds of multiple shoots which can be used for mass clonal propagation. The concentration of BAP is 50 mg/l and TDZ is 2 mg/l. The mix of BAP and TDZ solution was dropped on the surface of apical shoot. Figure 2 shows the “katak” forming many shoots.

They look healthy and can be used to produce mass clonal propagation of the A. konjac. In my opinion, shoots from “katak” can be used for explants of shoot apical meristem of A. konjac.