Mateus O Leite

doi:10.19080/JETR.2021.06.555689

Mini Review

IGF-1-PI3K-Akt-mTOR and Myostatin-SMAD3 Pathways Signaling for Muscle Hypertophy

Mateus O Leite, Thales M A Silva and Marco Machado*

¹ Universidade Iguaçu Campus V at Itaperuna, Brazil

² Fundação Universitária de Itaperuna, Brazil

Submission: October 16, 2021; Published: November 19, 2021

*Corresponding author: Marco Machado, Universidade Iguaçu Campus V at Itaperuna, BR356, Km02, Itaperuna, RJ, Brazil

How to cite this article: Leite M O, Silva T M A, Machado M. IGF-1-PI3K-Akt-mTOR and Myostatin-SMAD3 Pathways Signaling for Muscle Hypertophy. J Endocrinol Thyroid Res. 2021; 6(3): 555689. DOI: 10.19080/JETR.2021.06.555689

Abstract

The control of muscle mass depends mainly on the action of two signaling pathways: IGF-1 axis and Myostatin. While one (IGF-1) signals increases in protein synthesis, the other (myostatin) acts by inhibiting muscle growth. The interaction of these signaling pathways with other mechanisms determines muscle mass. Furthermore, an important player in the process are the satellite cells in a dependente or non-dependent way to hypertrophy process.

Keywords: Skeletal muscle; Muscle mass control; Muscle protein syntesis

Introduction

Its described that the size of skeletal muscle is dependent upon the kinetic processes of muscle protein synthesis (MPS) and muscle protein breakdown (MPB), the difference between which (MPS minus MPB) dictates net protein balance [1,2]. A positive protein balance (when MPS is greater than MPB) determines the increase in skeletal muscle fiber volume called hypertrophy. In recent decades, studies in molecular biology and biochemistry have shown two intracellular pathways to control the size of muscle fibers [3-5]. The first of them positively regulates muscle growth, comprising a cascade that includes the growth factor IGF-1. The second pathway is an inhibitory mechanism. It down regulate muscle growth and includes myostatin [3-7]. The purpose of this work is to show that the signaling for muscle hypertrophy is done via activation or not of satellite cells.

Discussion

It has been showed that the inactivation of IGF-1 in the skeletal muscle of animals impairs growth and reduces the number and size of muscle fibers. On the other side, an overexpression of IGF-1 promotes muscle hypertrophy, in a process dependent on the activation of the PI3K-Akt-mTOR cascade [8-10]. Akt phosphorylation is able to increase protein synthesis, through the activation of mTOR, which interacts with other proteins, to form complexes mTORC1 with a raptor subunit and mTORC2 with a rictor subunit [10,11](Figure 1).

Amino acids are capable of activating mTOR [4,12]. The effect of amino acids in the regulation of protein synthesis seems to be mediated mainly by leucine, through a mechanism that can be via insulin or not. It is important to highlight that although amino acids are fundamental as a substrate for protein synthesis, the simple increase in intake does not induce hypertrophy per se [13]. Based upon animal and cell culture studies, only leucine is required to induce protein synthesis and mTOR signaling, however, in vivo human studies are needed to validate this hypothesis [14].

Knock-out animals for mTOR, present reduced postnatal muscle growth and expressive reduction in size only of the fast-twitch fibers with preservation of the slow ones despite severe myopathy [15]. Despite the activation of two complexes associated with mTOR, complex 1 has a more important role in protein synthesis, being also the target of the inhibitory potential of rapamycin, whose treatment inhibits development, muscle regeneration and compensatory hypertrophy caused in experimental models by eliminating synergists [11,15].

As showed, although mTORC1 is capable of influencing the 4E-BP1 (eukaryotic translation initiation factor) and S6K1 (S-6 Kinase 1) factors, only the latter is essential for muscle hypertrophy and, in fact, its deletion it results in atrophy and prevents the anabolic effect of the constitutive activation of Akt [5,15]. It has also been shown that the protein AMP kinase (AMPK), activated when intracellular energy levels are decreased, is able to block the inducing effect of mTORC1 on muscle hypertrophy and AMPK-deficient mice demonstrate hypertrophy [16,17]. Despite this, other factors must be involved in the activation of hypertrophy independently of mTOR activation, as rapamycin is able to only partially inhibit the accumulation of ribosomal RNA necessary for protein synthesis and which normally occurs after activation of mTORC1 in conjunction with the synthesis of tRNA and nuclear RNA polymerases I, II and III [7].

Interestingly, it was affected that the transcription factor Yin-Yang (YY1) is able to physically interact with mTORC1 and mediate the expression of mTOR-dependent mitochondrial gene expression [7]. Apparently, mTORC1 seems to be able to induce YY1 phosphorylation, inducing the displacement of a transcription repressor complex of several genes that directly contributes to the expression of numerous genes involved in the insulin/IGF-1 signaling pathway, including IGF1, IRS1, IRS2, Akt1 and Akt2 [7,18]. In this context, the administration of rapamycin, by inhibiting mTORC1 and dephosphorylating YY1, recruits again the repressor complex of the promoter region of these genes and blocks its transcription [7].

A second muscle hypertrophy control pathway involves a myostatin, a member of the transforming growth factor superfamily (TGF-β). Myostatin is produced by skeletal muscle itself and negatively regulates muscle growth [16]. The exposure of purified myostatin inhibits the protein synthesis of cultured myotube cells and its systemic administration promotes muscle atrophy in mice [16]. The participation of myostatin in this phenomenon has been classically represented by the high levels of hypertrophy observed in several species of mammals that have mutations in the myostatin gene [18] (Figure 2).

A protein known as follistatin has also been identified, which is produced by skeletal muscle and capable of inhibiting the action of myostatin, in addition to binding and neutralizing the activity of activin-A, another member of the TGF-β family that it also acts as a negative regulator of muscle growth [19]. Follistatin has a more profound impact on hypertrophy than the myostatin gene deletion itself [20]. Myostatin and activin-A interact with each other and activate heterodimeric receptor in a process that antagonizes protein synthesis and muscle hypertrophy, and which can be inhibited by administering the soluble form of activin-A type II receptor (ACVR2B) [19]. The signal mediated by the myostatin/activin-A complex is believed to occur through phosphorylation and nuclear translocation of transcription factors SMAD2 and SMAD3 and formation of heterodimers with SMAD4. Although the targets of these factors are not yet known, it is speculated that they interfere negatively in the Akt-mTOR pathway [21,22].

In fact, muscle hypertrophy produced by ACVR2B transfection can be inhibited by rapamycin and that induced by follistatin is blocked by inactivation in different segments of the IGF-1/Akt/mTOR pathway [9,21]. Likewise, follistatin overexpression, induced by viral injection in skeletal muscle, increases Akt phosphorylation and signals the activation of protein synthesis, in a process dependent on mTOR activation and which is suppressed by exposure to rapamycin [7]. Apparently, inhibiting the interaction of SMAD3 with follistatin is critical for Akt-mTOR signaling even though, as demonstrated in other cell systems, SMAD3 is able to directly interact with Akt and suppress protein synthesis in that environment [21,23].

Such evidence demonstrates that there is communication between the myostatin/activin-A complex and the IGF-1 signaling pathway in order to control the degree of muscle fiber hypertrophy [24]. However, it has been shown that other pathways are also involved in the skeletal muscle hypertrophy process. These include serum responsive factors (SRFs), which are required to induce hypertrophy in synergists' ablation models, in a role mediated by interleukins 6 and 4 (IL-6 and IL-4) which act in a paracrine manner to induce proliferation and satellite cell fusion [25,26]. It has also been shown that SRFs activate Akt through the microRNA, miR-486, which is shown to be able to inhibit PTEN phosphatase, which negatively affects PI3K-Akt signaling [27].

Androgens and beta-2 adrenergic agonists are equally capable of activating the PI3K-Akt-mTOR pathway and contribute to increasing the protein synthesis process. In this sense, the administration of testosterone or mimetics that prioritize the development of male secondary sexual characteristics are capable of producing hypertrophy and animal models where there is a lack of testosterone, it reduces Igf-1 mRNA, Akt phosphorylation and rate of protein synthesis in processes that are reversed with nandrolone [19]. Similarly, clenbuterol or formoterol, beta-2 adrenergic agonists, are able to activate, through the increase of cyclic AMP, Akt phosphorylation, in a process that is blocked by rapamycin [28].

Another extracellular protein known as Wnt7a is capable of contributing to hypertrophy through activation of satellite cells and binding to the Fzd7 receptor, which is also involved in the activation of the PI3K-Akt-mTOR pathway [29]. This process seems to be enhanced by the positive impact of tensional changes in the sarcolemma and membrane caveoli, which contain an abundance of the nitric oxide synthetase enzyme in its neuronal isoform, which is abundant in skeletal muscle. It is believed that the released nitric oxide would be able to interact with superoxide anions produced by the contractile fiber mitochondria during exercise, and promote the synthesis of peroxynitrite, an aggressive reactive oxygen species that, however, has already been involved in the activation of vanilloid receptors Trpv1 present in the sarcoplasmic reticulum, and subsequent release of calcium ions. Thus, the moderate increase in the concentration of calcium ions in the cytosol would be able to positively influence numerous metabolic aspects and also activate mTOR to promote alterations in the protein synthesis process [14,19].

Mechanic stimuli in the membrane would also be able to activate mTOR through an Akt-independent pathway that involves the participation of phosphatidic acid and activated phospholipase D [7,30]. On the other hand, the fourth isoform of the transcription factor PGC-alpha was recently identified, whose isoform 1 is traditionally involved in mitochondrial biogenesis and is targeted by AMPK. Differently, PGCα-4 would be involved in increasing muscle mass and strength, being able to repress the action of myostatin and, in fact, in clenbuterol-induced hypertrophy it is abolished by deletion of the PGCα-4 gene [16].

Finally, although we have previously discussed the role of satellite cells in the hypertrophy process, it should be noted that muscle regeneration recaptures embryonic and neonatal myogenesis in a process that involves the activation, self-renewal, proliferation and fusion of myogenic and near stem cells always includes immune activation and inflammation [31]. Anyway, it is important to emphasize that muscle hypertrophy may exist even in the absence of significant activation of satellite cells and in fact, when this phenomenon is stimulated by clenbuterol, the muscle growth process does not involve satellite cell activation, as occurs in reintroduction of gravitational load, after a period of intentional removal to induce muscle atrophy [26,32].

Even in follistatin-induced hypertrophy signaling, satellite cells do not appear to play a role [15]. However, in the aforementioned experimental models, where synergists are intentionally removed from the limbs of animals to produce compensatory hypertrophy of the agonists, there is immediate proliferation and fusion of satellite cells, with well-evidenced increases in the number of myonuclei in a process that is also verified after eccentric contraction performed with high load and for a long time and capable of producing micro-lesions and localized inflammation [31]. Such experimental models are traditionally performed in rats and involve the removal of the gastrocnemius or tibialis anterior to observe, respectively, the hypertrophy of the soleus or extensor digitorum longus [33,34].

Likewise, it is well demonstrated that IGF-1-mediated hypertrophy involves the activation of satellite cells, and that the cytokines IL-6 and IL-4 are directly involved in muscle growth in experimental models involving overload [24]. Anyway, it is important to remember that even with the elimination of 90% of satellite cells through the use of toxins for PAX7, it is still possible to verify some muscular hypertrophy. It seems evident that, in mammals, there may be hypertrophy with or without the involvement of satellite cells [15,18]. In this context, acute and aggressive stimuli such as the elimination of synergists or intense exercise with a high eccentric component and presence of muscle damage, seem to necessarily involve the participation of satellite cells for regeneration and compensatory adaptive hypertrophy[35]. On the other hand, more gradual physical exercise stimuli and experimental models that replace the load on one or both limbs after intentional removal (functional overload model), the verified hypertrophy, does not seem to involve the activation of satellite cells, being exclusively associated to the increase of the protein synthesis process [36,37].

Conclusion

Based on the above, we can verify the central, but antagonistic, roles of IGF-1 and myostatin in controlling muscle hypertrophy. The factors shown effect their actions through complex intracellular signaling mechanisms. In addition, other mechanisms contribute to the control of hypertrophy, such as amino acids, androgens and inflammatory molecules. Satellite cells are not always involved in hypertrophy, and there is an activation of them mediated or not by IGF-1 and other factors.

JETR.MS.ID.555689

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