Antimalarial Effect and 0ther Properties of Hoslundia opposita - A Review
Sadri Abdullah Said*
School of Pharmacy, University of Nizwa, Oman
Submission: December 14, 2017; Published: January 08, 2018
*Corresponding author: Sadri Abdullah Said, School of Pharmacy, College of Pharmacy and Nursing, University of Nizwa, Oman, Tel: +968 2544 6423; Email: sadri@unizwa.edu.om
How to cite this article: Sadri Abdullah Said. Antimalarial Effect and 0ther Properties of Hoslundia opposita - A Review. Glob J Pharmaceu Sci. 2017; 4(3): 555636.DOI:10.19080/GJPPS.2017.04.555636
Abstract
Hoslundia opposita Vahl a plant widely distributed in Africa belongs to the family Lamiaceae and is the only species in the genus Hoslundia. Ethnobotanical and ethnopharmacological knowledge on H. oppositaas effective antimalarial species resemble those of cinchona from South America and qinghao from China. Preliminary biomedical study on H. opposita afforded potent antimalarial metabolites. Extending the biomedical work on this plant as well as on other antimalarial plants from Africa might see the continent taking its turn in providing a powerful antimalarial agent after quinine from America and artemisinin from Asia. This paper gives a brief review of H. opposita and is a call to researchers to investigate this plant and other potent antimalarial species growing in Africa.
Keywords: Antimalarial; Hoslundia opposita; Africa; Ethnobotany; Ethnopharmacology
Introduction
Malaria has been affecting human population for thousands of years and is still among the largest world health burden. Efforts to control malaria can be dated back as early as human started to be affected by this malady but was intensified in the 19th and 20th centuries by wars and colonial expansions in the world tropic and subtropical regions [1]. Top on early endeavor to control malaria was chemotherapy which was later aided by use of insecticide such as DDT to fight mosquito of the plasmodium species that was recognized as the vector for spread of malaria. Eradication of malaria in the developed world due to these efforts and the escalation of costs of development of new pharmaceuticals led to decline in interest in development of new antimalarials since 1970s. However, in the turn of the millennium there has been renewed concern for malaria as global problem and its control has been the focus of many declarations including, United Nations Millennium Declaration, The Abuja Declaration and the Plan of Action on Roll Back Malaria and many more [2]. Notwithstanding, great achievement of those goals in reducing malaria cases and deaths in the last fifteen years, malaria is still a large global health burden. It is reported that 212 million cases of malaria occurred in 2015 and 429,000 individuals died consequently [3]. Thus new innovations are still required in control of this deadly disease.
Chemotherapy has been the mainstay of malaria control. Based on ethnomedical history, terrestrial plants have been the major source of malaria chemotherapeutics. Herbal remedies using cinchona bark and qinghao were used effectively to treat malaria for many years before the discovery of quinine from cinchona bark in 1820 and artemisinin in 1972 [1]. Discovery and development of drugs based on these natural products has previously led to The Nobel Prize in Physiology or Medicine [4]. In addition to these natural products the use of a number of other drugs such as chloroquine has been effective arsenal to fight malaria before the parasite developed resistance to many of the previous regimens.
At present ACT has been the only effective therapy for treatment of malaria and recently there has been reports on occurrence of artmisenin resistant strains. There is therefore a need to initiate investigation aimed on discovery of new antimalarial agents. H. opposita is a shrub which is widely used in Africa for treatment of all forms of malaria including chronic variants. Preliminary biomedical study on this species showed the presence of potent antimalarial metabolites with chemical structures that differ markedly from previously known antimalarials. Like cinchona from South America and qinghao from Asia, H. opposita might be among potential sources of new antimalarial from Africa. This paper constitutes a general review of this species and its preliminary investigations as a source of new malaria chemotherapeutic agents.
Plant Description
Hoslundia opposite Vahl is an herbaceous perennial shrub that is widely distributed in tropical and subtropical open lands of Africa. It grows up to 3-5m high with slender glabrous or slightly pubescent branchlets. Leaves opposite, petioled, oblong or oblong-lanceolate, acute, crenate at the base, dentate, 8-10cm long, glabrous above slightly pubescent beneath. Inflorescence an ample lax deltoid terminal panicle; branchlets pubescent; bracts very small; pedicels as long as the flower-calyx. Flowers- calyx pubescent, 0.21cm long teeth deltoid. Fruit-calyx globose, red, edible [5]. It belongs to the family, Lamiaceae and is the only species in the genus Hoslundia. However, in the literature it is synonymously identified as Hoslundia paniculata, Hoslundia cornutioides, Hoslundia opposite, Clerodendrum micranthum, Hoslundia verticillata, Hoslundia sp., Orthosiphon physocalycinus, Micranthes menthoides, Hoslundia decumbens and Hoslundia unrecorded [6-8]. H. opposite Vahl is taxonomical classified under Kingdom: Plantae, Phylum: Magnoliophyta, Class: Magnoliopsida, Order: Lamiales, Family: Lamiaceae, Genus: Hoslundia and the Species: Hoslundiaopposita.
Traditional Uses
H.opposita is a popular herb in Sub-Saharan Africa used as remedy for various diseases. It is reported to be used for treatment of resistant malaria, fever, stomach pain, cough, wounds, sores, chest pain, liver damage, gonorrhea, cystitis, conjunctivitis, epilepsy, stomach trouble, and mental disorders as well as prevention and as an antidote for snake bites. There are several reports in the literature describing the use of different part of H. opposita in African traditional medicine; the following paragraphs are briefly summarizing the use of H. opposita in treatment of various diseases in different part of Africa. In Tanzania, one of East African countries, infusion of root bark of H. opposita has been locally used in Tanzania to fight chloroquine resistant strains, especially before the advent and commercialization of artemisinin based medicines. Furthermore, different parts of H. opposita are traditionally used for gonorrhoea, cystitis, coughs, wounds, liver disease, blennorrhoea and hookworms as well as for control of epilepsy in Tanzania [9-11].
In the neighboring Kenya and Uganda, concoction or infusion of H. opposita are also used as antimalarials. Other uses of this species in Kenya include remedies for colds, coughs, sore throat, gonorrhea, convulsion, stomach pains, constipation, ringworms and parasitic skin infection [12,13]. While in Uganda it is used for colic pain, enema, headache, miscarriage splenomegaly, stomachache syphilis uterine pains, and wounds [14,15]. Furthermore, preparations of H. opposita are used in Uganda traditional medicine for postnatal health, vomiting during fever and jaundice [16]. In Southern part of the continent there is no report on the use of H. opposite as antimalarial. However, the species has been used as remedy for various complications. For instance, in Zimbabwe extract from the leaves of H. opposita is dropped into eyes as cataract medicine and the root infusions are used to treat fits and epilepsy [17,18].
In Western Africa countries such as Cameroon, infusions from leaves of H. opposita are reported to be used as a purgative, diuretic, febrifuge, antibiotic, and antiseptic as well as for anaemia and skin diseases by traditional healers [19]. In Eastern Nigeria H. opposita is widely used in folk medicine for the treatment of cough, chest pain, fever, hookworm, stomach disorders, wounds, liver diseases and mental disturbances [20]. Leaves or root of H. opposita are used oral or topical to treat chronic and deep wounds, stomach ulcer, as well as dermatitis in some regions of Ghana [21,22]. Furthermore, Essential oils from the leaves of H. opposita are extensively used as insect repellents [23]. Leaves infusion is used as antidiabetic in Guinea [24].
Pharmacological Properties
Biomedical investigations of organic extracts obtained from various parts of H. opposita have revealed that this plant possesses diversified pharmacological activities. Reported biological activities include antimalarial, antimicrobial, antioxidant, CNS depression, hepatoprotective, and insecticidal properties. The evaluated biological properties are further described below.
Antimalarial
Organic extracts from root bark of H. opposita collected from Tanzania were shown to have high in vitro antimalarial activity against multidrug resistant K1 strain of Plasmodium falciparum Hexane extract was the most effective(IC50=5.6μg mL-1). It also showed a 26% inhibition of growth of P. berghei in mice, at a daily dose of 190mg kg-1 body weight, for four days [25]. Quinonesisolated from these extracts showed significant in vitro activity against the multidrug-resistant K-1 strain and the chloroquine-sensitive NF54 strain of P. falciparum, with IC50 values of 0.4 and 0.22μg mL-1, respectively [10]. Furthermore, different parts of H. opposita collected in Kenya displayed strong antiplasmodial activity against CQ sensitive (D6) and CQ resistant (W2) Plasmodium falciparum strains with IC50 values2-4μg mL-1[12].
Antimicrobial
Crude extracts of the entire plant collected from the coast of Tanzania showed strong antibacterial activity against a series of gram positive and gram negative bacteria [9]. However, ethanolic extract of H. opposita collected from hinterland of Tanzania showed poor antimicrobial activity [26]. Extracts from H.opposita collected from Mozambique demonstrated good antimicrobial activity against strains of Mycobacterium tuberculosis. Subsequent phytochemical investigation on these extracts afforded isolation of several compounds including euscaphic acid and 5,7-dimethoxy-6-methylflavone. The former compound was found to inhibit the growth of drug-sensitive M. tuberculosis at concentration of 50μg mL-1 while the latter was shown to inhibited the HIV-1 reverse transcriptase enzyme by 52% at a concentration of 100 μg mL-1 [27].
A part from that, essential oil from leaves of H.opposita collected from Central Africa showed significant antimicrobial activity against Aspergillus niger, Acinetobacter calcoacetica,Brochothrix thermosphacta and Flavobacterium suaveolens.[28] . Moreover, the results on the investigation of antimicrobial activities of aqueous, methanol and ethyl acetate extracts of leaves of H.opposita showed antibacterial activities against strains of gram positive and negative bacteria at concentration of 20μg/mL with zone of inhibition ranging from 6.5-11.0mm[29] .
Insecticidal
Crude methanolic extract from leaves of H. opposita as well as pet.ether, ethyl acetate and aqueous fractions partitioned from the methanolic extract were found active against the larvae of the cattle tick, Amblyomma variegatum, However, all extracts were less potent than the control, malathion. LC50 values of the active extracts were more than 500 times higher than that displayed by malathion (1.14*10-4mg/ml). Furthermore, ursolic acid isolated from ethyl acetate fraction showed weaker acaricidal effects (LC50=1.13mg/ml). This result is an indication that the overall acaricidal effect of H. opposita might be due to synergistic effect of individual principles within the extracts [23]. In a similar investigation extracts from leaves, stem and root barks of H. opposita collected from Tanzania showed significant larvicidal activity against A. gambiae larvae under laboratory trials [30]. Furthermore, essential oil from H. opposita showed insecticidal activity against the red follower beetle Tribolium castaneum [31].
Hepatoprotective
Organic extract of H. opposita showed hepatoprotective effect against carbon tetrachloride (CCl4) and paracetamol- induced liver damage in rats. Treatment of affected animals with methanol extract and methanol and ethyl acetate fractions of H. opposita (100mg/kg) ameliorated the effects of the hepatoxins and significantly reduced the elevated levels of the biochemical marker enzymes such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP) as well as bilirubin. The extracts showed good toxicity profile with an LD50 value above 5000mg/kg for the methanol extract [20].
Antioxidant
In determination of antioxidant activity of some wound healing plants growing in Ghana Agyare et al. [21] found that ethanolic extract from leaves of H. opposita has strong DPPH radical scavenging activity (RC50=9.8μg/ml) [21]. However, ethanol extract from stem of H. opposita collected from Zimbabwe showed poor antioxidant activity in similar investigation conducted by Muchuweti & coworkers [32].
CNS Depression
The chloroform extract of the dried root of H. opposita showed depression of central nervous system (CNS). It significantly potentiated the phenobarbitone sleeping time in mice and produced a 60% protection against leptazol-induced convulsion [33]. In addition, ethanolic extracts from leaves of H. opposita collected from South Africa showed dose-dependent GABAergic activity in the GABAA-benzodiazepine receptor binding assay [34,35].
Chemical Constituents
Basic phytochemical screenings of organic extracts from H. opposita have revealed the presence of secondary metabolites that could be grouped as sterols, terpenoids, saponins, flavonoids and tannins [13,20]. Individual natural products that have so far been isolated from various parts of H. opposita include terpenoids, flavonoids and quinones. Four quinones, including the hosloppones, 3-O-benzoylhosloppone (Figure 1), 3-O-cinnamoylhosloppone (Figure 2), as well as 3- O-benzoylhinokiol (Figure 3) and 3-O-benzoylhosloquinone (Figure 4) were afforded from root bark of H. opposita collected from Tanzania. Hosloppone, Figure 1 showed in vitro antimalarial activity against the multidrug-resistant K-1 strain and the chloroquine-sensitive NF54 strain of P falciparum (IC50=0.4 and 0.22μg mL-1, respectively). The remaining compounds were not assayed because only limited quantity was available [10]. Based on ethnobotanical and ethnopharcological use of H. opposita it would have been interesting to evaluate antimalarial property of these compounds as well as to prepare and evaluate extra analogs of compound Figure 1 to reveal SAR of the hosloppones for further development of new antimalarials.
In 2009 Mujovo [27] reported isolation of three compounds including, euscaphic acid (Figure 5), hoslunddiol (Figure 6) and 5,7-dimethoxy-6-methylflavone (Figure 7) from leaves of H. opposita collected from Mozambique. Euscaphic (jacarandic acid, Figure 5) acid was found active against drug sensitive strains of Mycobacterium tuberculosis (MIC~50mcg/ml). Compound 7 inhibited HIV-1 reverse transcriptase enzyme by 52% at 100μg/ ml [30]. More recently, Annan and his group reported isolation of ursolic acid (Figure 8), from the leaves of H. opposita [23].
Conclusion
Ethnopharmacological information as well as preliminary biomedical data on Hoslundia opposita growing in East Africa shows that this species might be a potential source of new chemical entities with desirable pharmacological properties for development of new malaria chemotherapeutic agents.
References
- Rosenthal PJ (2001) Antimalarial Chemotherapy Mechanisms of Action, Resistance and New Directions in Drug Discovery. Br J Clin Pharmacol 52(4): 464.
- WHO (2015) World malaria Report.
- WHO (2016) World malaria Report.
- William Campbell C, Satoshi Ōmura, Youyou Tu (2015) The Nobel Prize in Physiology or Medicine 2015.
- Oliver D (1868) Flora of tropical Africa. Lovell Reeve and co publishers, London, England, pp. 377.
- http://plants.jstor.org/taxon/vern.names/Hoslundia.
- Iwu MM (2014) Handbook of African Medicinal Plants. (2nd edn), CRC Press, Florida, USA, pp. 237.
- Quattrocchi U (2012) CRC World Dictionary of Medicinal and Poisonous Plants: Common Names, Scientific Names, Eponyms, Synonyms, and Etymology (5 Volume Set). CRC press, Florida, USA, p. 3960.
- Khan MR, Nkunya MHH (1991) Antimicrobial activity of Tanzanian traditional medicinal plants In Ministry of Health, Tanzania Proceedings of an international conference on traditional medicinal plants. Dar es Salaam University Press, Tanzania, p. 391.
- Achenbach H, Waibel R, Nkunya MHH, Weenen H (1992) Antimalarial compounds from Hoslundiaopposita. Phytochemistry 31: 3781-3784.
- Moshi MJ, Kagashe GAB, Mbwambo ZH (2005) Plants used to treat epilepsy by Tanzanian traditional healers Journal of Ethnopharmacology 97(2): 327-336.
- Gathirwa JW, Rukunga GM, Mwitari PG, Mwikwabe NM, Kimani CW, et al. (2011) Traditional herbal antimalarial therapy in Kilifi district, KenyaJournal of Ethnopharmacology 134(2): 434-442.
- Okach DO, Nyunja ARO, Opande G (2013) Phytochemical screening of some wild plants from Lamiaceae and their role in traditional medicine in Uriri District-Kenya International Journal of Herbal Medicine 1(5): 135-143.
- Asiimwea S, Namutebib A, Borg-KarlssoncAnna K, Mugishaa MK, Oryem-Origaa H (2014) Documentation and consensus of indigenous knowledge on medicinal plants used by the local communities of western Uganda. J Nat Prod Plant Resour 4(1): 34-42.
- Stangeland T, Alele PE, Katuura E, Lye KA (2011) Plants used to treat malaria in Nyakayojo sub-county, western Uganda. J Ethnopharmacology 137(1): 154-166.
- Auerbach BJ, Reynolds SJ, Lamorde M, Merry C, Kukunda-Byobona C (2012) Traditional Herbal Medicine Use Associated with Liver Fibrosis in Rural Rakai, Uganda. PLoS ONE 7(11): e41737.
- Maroyi A (2013) Traditional use of medicinal plants in south-central Zimbabwe: review and perspectives. Journal of Ethnobiology and Ethnomedicine 9: 31
- Stafford GI, Pedersen ME, van Staden J, Jäger AK (2008) Review on plants with CNS-effects used in traditional South African medicine against mental diseases. Journal of Ethnopharmacology 119(3): 513537.
- Koné WM, Koffi AG, Bomisso EL, Tra Bi FH (2012) Ethnomedical study and iron content of some medicinal herbs used in traditional medicine in coted'ivoire for the treatment of anaemia. Afr J Tradit Complement Altern Med 9(1): 81-87.
- Akah PA, Odo CI (2010) Hepatoprotective effect of the solvent fractions of the stem of Hoslundia opposita Vahl (Lamiaceae) against carbon tetrachloride- and paracetamol-induced liver damage in rats. Int J Green Pharm 4(1): 54-58.
- Agyare C, Asase A, Lechtenberg M, Niehues M, Deters A, et al. (2009) An ethnopharmacological survey and in vitro confirmation of ethnopharmacological use of medicinal plants used for wound healing in Bosomtwi-Atwima-Kwanwoma area. Ghana Journal of Ethnopharmacology 125(3): 393-403.
- Pesuwa GA, Cutler RR, Humber DP (2008) Antibacterial activity of plants used in traditional medicines of Ghana with particular reference to MRSA. Journal of Ethnopharmacology 116(1): 102-111.
- Annan K, Jackson N, Dickson RA, Sam GH, Komlaga G (2011) Acaricidal effect of an isolate from Hoslundia opposita vahl against Amblyomma variegatum (Acari:Ixodidae). Pharmacognosy Res 3(3): 185-188.
- Diallo A, Traore MS, Keita SM, Balde MA, Keita A, et al. (2012) Management of diabetes in Guinean traditional medicine: An ethnobotanical investigation in the coastal lowlands. Journal of Ethnopharmacology 144(2): 353-361.
- Weenen H, Nkunya MH, Bray DH, Mwasumbi LB, Kinabo LS, et al. (1990) Antimalarial activity of Tanzanian medicinal plants. Planta Med 56(4): 368-370.
- Maregesi SM, Pieters L, Ngassapaa OD, Apers S, Vingerhoets R, et al.(2008) Screening of some Tanzanian medicinal plants from Bun da district for antibacterial, antifungal and antiviral activities. Journal of Ethnopharmacology 119(1): 58-66.
- Mujovo SF, Hussein AA, Meyer JJM, Fourie B, Muthivhi T, et al. (2008) Bioactive compounds from Lippia javanica and Hoslundia opposite. Natural Product Research 22(12): 1047-1054.
- Gundidza GM, Deans SG, Svoboda KP, Mavi S (1992) Antimicrobial activity of essential oil from Hoslundiaopposita. Cent Afr J Med 38(7): 290-2933.
- Ojo OO; Anibijuwon II (2011) Studies on extracts of three medicinal plants of South-western Nigeria: Hoslundia opposita, Lantana camara and Cymbopogoncitratus. The Free Library, Pennsylvania.
- Kihampa C, Joseph CC, Nkunya MHH, Magesa MS, Hassanali A, et al.(2009) Larvci dal and IGR activity of extract of Tanzanian plants against malaria vector mosquitoes. J Vector Borne Diseases 46: 145152.
- Babarinde SA, Akinyemi AO, Usman LA, Odewole AF, Sangodele AO, et al. (2014) Toxicity and repellency of Hoslundia opposita Vahl (Lamiaceae) leaves' essential oil against rust-red flour beetle, Tribolium castaneum Herbst (Coleoptera: Tenebrionidae). Natural Product Research 28(6): 365-371.
- Muchuweti M, Nyamukonda L, Chagonda LS, Ndhlala AR, Mupure C, et al. (2006) Total phenolic content and antioxidant activity in selected medicinal plants of Zimbabwe International. Journal of Food Science and Technology 41 (Suppl 1): 33-38.
- Olajide OA, Awe SO, Makinde JM (1999) Central nervous system depressant effect of Hoslundia opposite Vahl. Phytotherapy Research 13(5): 425-426.
- Risa J, Risa A, Adsersen A, Gauguin B, Stafford GI, et al. (2004) Screening of plants used in southern Africa for epilepsy and convulsions in the GABAA-benzodiazepine receptor assay Journal of Ethnopharmacology 93(2-3): 177-182.
- Risa A, Risa J, Adsersen A, Stafford GI, van Staden J, et al. (2004) Acetylcholinesterase inhibitory activity of plants used as memory- enhancers in traditional South African medicine South African Journal of Botany 70: 664-666.