Correction of Platelet Activity in New-Born Calves with Iron Deficit as a Result of Ferroglukin, Polyson and Cresacin

During our research it was established that new-born calves with iron deficit also have lowering of plasma antioxidant protectability, intensification of lipids’ peroxidation processes, increase of thrombocytes’ hemostatic activity and blood coagulant system, and at the same time – decrease of vascular wall’s ability to bound it. As a result of application of ferroglukin, polyson and cresacin combination to new-born calves with iron deficit we managed to get evident increase of blood plasma antioxidant protectability, significant decrease in it of lipids’ peroxidation processes at normalization of thrombocyte activity and positive dynamics of hemostasis vascular and plasma components.


Introduction
As the state of iron deficit is spread enough among new-born calves [1] and it is rather often accompanied by development of disturbances in hemostasis system [2] there is a great practical demand in their quick and effective removal among calves at farms. At the same time effective approaches aimed at simultaneous reduction of iron deficit and hemostasiopathy signs are still worked out unsatisfactorily [3].
That's why investigations led with new-born calves with the aim of finding approaches to early and effective hemostasiopathy correction on the model of iron deficit state keep their great scientific and practical significance. Worked out at the given state varients of evidence decrease of hemostasis disturbances can serve the basis for the following creation of correction complexes able to be effective in the field of hemostasiopathy reduction of new-born calves at many diseases. Great interest should exist to the evaluation of influence on the whole hemostasis system of the combination of traditionally applied at iron deficit ferroglukin [3], and earlier shown their high biological activity and ability to influence hemostasis system separate components metabolically active means-polyson [4] and cresacin [5].
In this connection we put the following aim for our investigation -to find the evidence of platelets activity correction of new-born calves with iron deficit with the help of ferroglukin, polyson and cresacin combination.

Materials and Methods
The work was fulfilled with 37 new-born calves having the signs of erythrogenesis and decrease of iron content in their organisms (serum iron 13,1±0,09mkmol/l, siderocytes 1 , 5 ± 0 , 0 5 % , h a e m o g l o b i n 9 8 , 2 ± 0 , 2 5 g / l , e r y t h r o c y t e s 4,2±0,18x10 12 /l). The control group contained 29 healthy newborn calves.
The state of lipids' peroxidation (LPO) in animals' plasma was found out according to the quantity in it of thiobarbituric acid-active products with the help of a set by the firm "Agat-Med" (Russia) and acylhydroperoxides with the account of antioxidant activity level of the liquid part of blood [6]. Thrombocytes' number in calves' blood was found out by their calculation in Gorjaev's chamber. Thrombocytes aggregation was registered by visual micro method [7] with some inductors: with ADP (0,5x10 -4 M), with thrombin (0,125un/ml), with collagen (dilution 1:2 of the main suspension), with rhystomicin (0,8mg/ml), with adrenalin (5x10 -6 M) in plasma with standardized quantity of thrombocytes in it (200x10 9 tr.).

Agricultural Research & Technology: Open Access Journal
The correction of iron deficit state of new-born calves was realized by ferroglukin intra muscularly, once from the calculation of 15mg of iron on 1kg of body mass, polyson 5mg/kg in the morning in the scheme of liquid feeding during 6 days and cresacin-every day 3mg/kg in the scheme of liquid feeding during 6 days beginning simultaneously with ferroglukin application. Evaluation of healthy animals' state was made two times-at their birth and on the 7 th day of life. Because of the absence of reliable differences between the results of both investigations control values of each index are presented by one figure-a simple average between them. Examination of calves having iron deficit was fulfilled twice -at their birth and on the next day after correction finish (the 7 th day of life). Statistical processing of received data was fulfilled by Student's t-criteria.
Correction realization of animals having at the beginning iron deficit was accompanied by invariability of thrombocytes' quantity in their blood and slowdown of thrombocytes' aggregation to the control level. Besides, most actively animals' thrombocytes responded by aggregation to collagen, ADF and rhystomicin, less actively -to thrombin and adrenalin addition into plasma (Table 1). Table 1: Parameters of hemostasis in newborn calves with iron deficiency treated with ferroglukin, polyson and cresacin. p -reliability of differences of indicators between the control and the initial state of the calves with iron deficiency, p1 -reliability of dynamics of indicators in calves with iron deficiency against the background of correction. Realization of genetically defined growth and development processes of living organisms takes place at constant influence on organism of numerous factors of environment and internal environment. Physiological peculiarities of their influence are mostly expressed by the optimum of living beings' blood content [8] especially as far as hemostasis system components' activity is concerned [9]. Besides, any disturbances in an organism are accompanied by negative dynamics of hematological indices including parameters of hemostasis system. It becomes clear, that in the basis of hemostasiopathy development in case of examined new-born calves we have not only iron deficit but also found during investigation depression of plasma antioxidant defiance which as previous works showed causes LPO activation in it. Increase of peroxidation in plasma damages structures of blood platelets and vessels and affects their functions. Found in new-born calves with iron deficit thrombocytes' aggregation acceleration points at the increase of their receptors' sensibility to stimulating influences from the outside. Besides, active Agricultural Research & Technology: Open Access Journal development of thrombocytes' aggregation in response to rhystomicin in case of calves with iron deficit should be regarded as consequence of their sensibility increase to Willybrand's factor. Besides, acceleration of thrombocytes' aggregation coming of these animals indirectly tells about the increase in their blood platelets of exchange processes of arachidonic acid with surplus thromboxan A2 formation [10].

Consider Indicators
Application of ferroglukin, polyson and cresacin combination made new-born calves with iron deficit state feel saturation of their organisms with iron, positive dynamics of red blood and common animals' state indices. Fulfilled impact on examined calves' organisms was accompanied by lowering of their LPO processes intensity in plasma what weakened its damaging influence on endothelium and liver thrombocytes [11]. Found normalization of thrombocytes aggregation of calves with iron deficit state after getting of ferroglukin, polyson and cresacin combination is mostly the consequence of these means combination positive impact on inner thrombocyte LPO, receptor and post receptor thrombocytes' functioning mechanisms. Developing in these conditions time increase of thrombocytes aggregation coming in response to rhystomicin pointed at lowering in these calves' blood of adhesion cofactor -Willybrand's factor [12].

Conclusion
New-born calves having iron deficit are characterized by lowering of blood plasma antioxidant defence, intensification in it of LPO processes, increase of thrombocyte hemostatic activity. With the help of application to new-born calves with iron deficit of the combination of ferroglukin, polyson and cresacin we can really strengenplasma antioxidant defence, weaken LPO activity in it, normalize thrombocyte activity.